ML 334
Modify Date: 2024-01-08 11:13:11
ML 334 structure
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Common Name | ML 334 | ||
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| CAS Number | 1432500-66-7 | Molecular Weight | 446.50 | |
| Density | 1.354±0.06 g/cm3(Predicted) | Boiling Point | 693.1±55.0 °C(Predicted) | |
| Molecular Formula | C26H26N2O5 | Melting Point | N/A | |
| MSDS | N/A | Flash Point | N/A | |
Use of ML 334ML334 is a potent, cell permeable activator of NRF2 by inhibition of Keap1-NRF2 protein-protein interaction. ML334 binds to Keap1 Kelch domain with a Kd of 1 μM. ML334 stimulates NRF2 expression and nuclear translocation and induces antioxidant response elements (ARE) activity[1][2]. |
| Name | ML334 |
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| Description | ML334 is a potent, cell permeable activator of NRF2 by inhibition of Keap1-NRF2 protein-protein interaction. ML334 binds to Keap1 Kelch domain with a Kd of 1 μM. ML334 stimulates NRF2 expression and nuclear translocation and induces antioxidant response elements (ARE) activity[1][2]. |
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| Related Catalog | |
| Target |
Ki: 1 μM (Keap1)[2] |
| In Vitro | ML334 (LH601A; 50-100 µM; 6-16 hours; HEK293 cells) treatment increases NQO1 and TRX1 mRNAs between 2- and 3-fold at both 6 and 16 h. And also enhances HO-1 mRNA expression between 4- and 7-fold at 6 h, with lesser fold-changes at 16 h[1]. ML334 (LH601A; 50-100 µM; 16 hours; HEK293 cells) treatment induces HO-1 and TRX1 proteins in HEK293 cells[1]. ML334 (LH601A) stimulates NRF2 expression and nuclear translocation in HEK293 cells[1]. RT-PCR[1] Cell Line: HEK293 cells Concentration: 50 μM or 100 µM Incubation Time: 6 hours, 16 hours Result: Increased NQO1 and TRX1 mRNAs between 2- and 3-fold at both 6 and 16 h. Enhanced HO-1 mRNA expression between 4- and 7-fold at 6 h. Western Blot Analysis[1] Cell Line: HEK293 cells Concentration: 50 μM or 100 µM Incubation Time: 16 hours Result: Enhanced HO-1 and TRX1 protein expression at 16 h. |
| References |
| Density | 1.354±0.06 g/cm3(Predicted) |
|---|---|
| Boiling Point | 693.1±55.0 °C(Predicted) |
| Molecular Formula | C26H26N2O5 |
| Molecular Weight | 446.50 |