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AC-DEVD-AFC

AC-DEVD-AFC用途

Ac-DEVD-AFC 是一种荧光底物 (λex=400 nm, λem=530 nm)。

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AC-DEVD-AFC名称

[ CAS 号 ]:
201608-14-2

[ 中文名 ]:
Ac-Asp-Glu-Val-Asp-7-氨基-4-三氟甲基香豆素

[ 英文名 ]:
Ac-Asp-Glu-Val-Asp-AFC

[英文别名 ]:

Ac-DEVD-AFC
MFCD01310970

AC-DEVD-AFC生物活性

[ 描述 ]:

Ac-DEVD-AFC 是一种荧光底物 (λex=400 nm, λem=530 nm)。

[ 相关类别 ]:

信号通路 >> 其他 >> 其他

研究领域 >> 癌症

染料试剂

多肽产品

[体外研究]

与Ac-DEVD-AFC一起温育1小时后,与对照相比,在4小时时观察到胱天蛋白酶-3活性显着增加。 Photofrin和LPLI组中caspase-3活性没有显着增加。 shRNA-BimL转染显着抑制了Ac-DEVD-AFC对caspase-3活化的反应[1]。

[细胞实验]

为了检测胱天蛋白酶-3活性，PBS洗涤悬浮在提取缓冲液[25mM HEPES（pH7.4），0.1％TritonX-100,10％甘油中的细胞沉淀（来自培养基或贴壁细胞）， 5mM DTT，1mM苯甲基磺酰氟，10mg / mL胃蛋白酶抑制剂和10mg / mL Leupeptin]并剧烈涡旋。将20μl提取物（相当于10％的样品）与caspase-3荧光底物Ac-DEVD-AFC在室温下以100μM终浓度温育，并通过监测荧光AFC的释放连续测量caspase-3活性。在37°C [1]。

[参考文献]

[1]. Wang X, et al. Involvement of Bim in Photofrin-mediated photodynamically induced apoptosis. Cell Physiol Biochem. 2015;35(4):1527-36.

[相关活性小分子]

AC-DEVD-AFC物理化学性质

[ 密度 ]:
1.5±0.1 g/cm3

[ 沸点 ]:
1152.7±65.0 °C at 760 mmHg

[ 分子式 ]:
C₃₀H₃₄F₃N₅O₁₃

[ 分子量 ]:
729.612

[ 闪点 ]:
650.9±34.3 °C

[ 精确质量 ]:
729.210510

[ PSA ]:
287.61000

[ LogP ]:
1.58

[ 外观性状 ]:
白色粉末

[ 蒸汽压 ]:
0.0±0.3 mmHg at 25°C

[ 折射率 ]:
1.575

[ 储存条件 ]:
−20°C

[ 水溶解性 ]:
H2O: 1 mg/mL

AC-DEVD-AFCMSDS

详细MSDS(安全技术说明书)

AC-DEVD-AFC安全信息

[ 个人防护装备 ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ 危险品运输编码 ]:
NONH for all modes of transport

[ WGK德国 ]:
3

AC-DEVD-AFC文献

A new water soluble MAPK activator exerts antitumor activity in melanoma cells resistant to the BRAF inhibitor vemurafenib

Biochem. Pharmacol. 95 , 16-27, (2015)

Nitrobenzoxadiazoles exert antitumor effects against vemurafenib-resistant melanoma cells by sustained activation of different MAPK pathways.

Inhibition of ovarian cancer cell growth by a novel TAK1 inhibitor LYTAK1.

Cancer Chemother. Pharmacol. 76 , 641-50, (2015)

Transforming growth factor-β-activating kinase 1 (TAK1) has been implicated in promoting ovarian cancer progression. Here, we evaluated the anti-ovarian cancer effect of LYTAK1, a novel and specific T...

AdipoRon, the first orally active adiponectin receptor activator, attenuates postischemic myocardial apoptosis through both AMPK-mediated and AMPK-independent signalings.

Am. J. Physiol. Endocrinol. Metab. 309 , E275-82, (2015)

Adiponectin (APN) is a cardioprotective molecule. Its reduction in diabetes exacerbates myocardial ischemia/reperfusion (MI/R) injury. Although APN administration in animals attenuates MI/R injury, mu...

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