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海美溴铵

海美溴铵用途

Hexadimethrine bromide 是一种聚合物,可增强逆转录病毒转导。

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海美溴铵名称

[ CAS 号 ]:
28728-55-4

[ 中文名 ]:
海美溴铵

[ 英文名 ]:
Hexadimethrine bromide

[英文别名 ]:

Hexadimethrine bromide,Polybrene
HEXADIMETHRINE BROMIDE
MFCD00133397

海美溴铵生物活性

[ 描述 ]:

Hexadimethrine bromide 是一种聚合物,可增强逆转录病毒转导。

[ 相关类别 ]:

信号通路 >> 其他 >> 其他

研究领域 >> 癌症

[体外研究]

Hexadimethrine bromide在慢病毒转导过程中抑制人间充质干细胞增殖。溴化己二甲铵在低浓度下被认为是无毒的,但已发现在浓度大于10μg/ mL的某些细胞类型中,细胞增殖会对细胞增殖产生负面影响。当在显微镜下观察时,暴露于溴化己二甲铵的胰蛋白酶消化的细胞明显更大[1]。

[细胞实验]

将胰蛋白酶消化的细胞洗涤并重悬浮于2×10 4细胞/ mL的培养基中，有或没有rhFGF-2（终浓度= 10ng / mL，PeproTech）和有或没有溴化己二甲铵，最终溴化己二甲铵浓度为1,4或8μg/ mL。将不同条件接种于96孔板中，每孔50μL，1×103个细胞，一式三份，并在37℃，5％CO 2下培养。在6,9或24小时后，更换培养基并随后用50μL±FGF-2培养基（10ng / mL）每3-4天发生培养基变化。通过除去培养基并将平板置于-80℃冰箱中直至分析当天，在第4,7,14和21天收获平板。然后在孔上进行CyQUANT测定[1]。

[参考文献]

[1]. Lin P, et al. Polybrene inhibits human mesenchymal stem cell proliferation during lentiviral transduction. PLoS One. 2011;6(8):e23891.

[相关活性小分子]

海美溴铵物理化学性质

[ 沸点 ]:
209.5ºC at 760 mmHg

[ 分子式 ]:
C₁₅H₃₆Br₂N₂

[ 分子量 ]:
404.27

[ 闪点 ]:
73.9ºC

[ 外观性状 ]:
白色至灰白色粉末

[ 蒸汽压 ]:
0.202mmHg at 25°C

[ 储存条件 ]:
2-8℃

海美溴铵MSDS

详细MSDS(安全技术说明书)

海美溴铵毒性和生态

CHEMICAL IDENTIFICATION

RTECS NUMBER :: TQ2655000

CHEMICAL NAME :: Poly((dimethyliminio)hexamethylene(dimethylimino)trim ethylene dibromide)

CAS REGISTRY NUMBER :: 28728-55-4

LAST UPDATED :: 199703

DATA ITEMS CITED :: 4

WISWESSER LINE NOTATION :: /E3E &1N1&5N1&1/

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 20 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

REFERENCE :: USXXAM United States Patent Document. (U.S. Patent Office, Box 9, Washington, DC 20231) Volume(issue)/page/year: #4013507

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: >1 gm/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

REFERENCE :: USXXAM United States Patent Document. (U.S. Patent Office, Box 9, Washington, DC 20231) Volume(issue)/page/year: #4013507

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intraperitoneal

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 30 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

REFERENCE :: RPTOAN Russian Pharmacology and Toxicology (English Translation). Translation of FATOAO. (Euromed Pub., 33, Woodlands Rd., Surbiton, Surrey, UK) V.30- 1967- Volume(issue)/page/year: 37,267,1974

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 28 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

REFERENCE :: USXXAM United States Patent Document. (U.S. Patent Office, Box 9, Washington, DC 20231) Volume(issue)/page/year: #4013507

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海美溴铵安全信息

[ 符号 ]:

GHS07

[ 信号词 ]:
Warning

[ 危害声明 ]:
H302

[ 个人防护装备 ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ 危害码 (欧洲) ]:
Xn: Harmful;

[ 风险声明 (欧洲) ]:
R20/21/22

[ 危险品运输编码 ]:
UN 2811

[ RTECS号 ]:
TQ2655000

[ 包装等级 ]:
III

[ 危险类别 ]:
6.1(b)

海美溴铵文献

NSC-87877 inhibits DUSP26 function in neuroblastoma resulting in p53-mediated apoptosis.

Cell Death Dis. 6 , e1841, (2015)

Dual specificity protein phosphatase 26 (DUSP26) is overexpressed in high-risk neuroblastoma (NB) and contributes to chemoresistance by inhibiting p53 function. In vitro, DUSP26 has also been shown to...

Polyomavirus T antigens activate an antiviral state.

Virology 476 , 377-85, (2015)

Ectopic expression of Simian Virus 40 (SV40) large T antigen (LT) in mouse embryonic fibroblasts (MEFs) increased levels of mRNAs encoding interferon stimulated genes (ISGs). The mechanism by which T ...

Polybrene: Observations on cochlear hair cell necrosis and minimal lentiviral transduction of cochlear hair cells.

Neurosci. Lett. 600 , 164-70, (2015)

Polybrene is widely used to enhance viral transduction; however, little is known about the utility thereof, in enhancing lentiviral transduction of cochlear cells. In the present study, we examined th...

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