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凝血酶

凝血酶用途

主要用于局部止血药物。

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凝血酶名称

[ CAS 号 ]:
9002-04-4

[ 中文名 ]:
凝血酶

[ 英文名 ]:
Thrombin

[英文别名 ]:

EINECS 232-648-7
MFCD00166053

凝血酶生物活性

[ 描述 ]:

Thrombin 是一种胰蛋白酶样别构丝氨酸蛋白酶,在凝血连锁反应中起作用。

[ 相关类别 ]:

信号通路 >> 代谢酶/蛋白酶 >> 凝血酶

研究领域 >> 神经疾病

[体外研究]

凝血酶是已知在凝血级联中具有基本作用的丝氨酸蛋白酶。此外,凝血酶还通过一个独特的蛋白酶激活受体(PARS)家族介导几种细胞过程,其中PAR-1是原型。凝血酶和PAR-1在脑中高度表达并调节突触传递和可塑性[1]。凝血酶属于胰凝乳蛋白酶家族,并且通过凝血酶原酶复合物蛋白水解切割其无活性前体凝血酶原而产生。凝血酶介导纤维蛋白原转化为血液凝块的主要成分-纤维蛋白。此外,凝血酶激活血液凝固因子V,VIII,XI,XIII和抗凝血蛋白C [2]。

[参考文献]

[1]. Golderman V, et al. Thrombin and the Protease-Activated Receptor-1 in Organophosphate-Induced Status Epilepticus. J Mol Neurosci. 2018 Dec 4.

[2]. Krenzlin H, et al. The Importance of Thrombin in Cerebral Injury and Disease. Int J Mol Sci. 2016 Jan 11;17(1).

[相关活性小分子]

凝血酶物理化学性质

[ 外观性状 ]:
powder | white

[ 储存条件 ]:
−20°C

[ 水溶解性 ]:
H2O: 1 mL/vial clear to slightly hazy, colorless solution

凝血酶安全信息

[ 符号 ]:

GHS07, GHS08

[ 信号词 ]:
Danger

[ 危害声明 ]:
H315-H319-H334-H335

[ 警示性声明 ]:
P261-P305 + P351 + P338-P342 + P311

[ 个人防护装备 ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ 危害码 (欧洲) ]:
Xn,B,Xi

[ 风险声明 (欧洲) ]:
36/37/38-42

[ 安全声明 (欧洲) ]:
22-24/25-36/37-26-24

[ 危险品运输编码 ]:
NONH for all modes of transport

[ WGK德国 ]:
2

[ RTECS号 ]:
XO8950000

凝血酶制备

1.取猪血2kg，加入柠檬酸钠溶液200ml（内含柠檬酸钠38g），混匀，离心分离。取上清液血浆，加入蒸馏水稀释，用300ml的1%乙酸调节pH值为5.3，待沉淀完全，收集沉淀，得凝血酶原。取凝血酶原，在25～30℃下，加入氯化钠溶液使其溶解，再在每升溶液中加入15g氯化钙，充分搅拌后，冷室静置1h，离心分离。上清液中加冷冻丙酮，搅拌均匀后，静置过夜。过滤，沉淀用乙醚洗涤，干燥，得凝血酶粗品。粗品中加入氯化钠溶液使其溶解，在0℃放置6h，过滤。滤液再用乙酸调节pH=5.5，离心分离，然后取上清液加入2倍量冷冻丙酮，静置2h，待沉淀完全后，离心分离。沉淀再分别用无水乙醇、乙醚洗涤，真空干燥，可制得凝血酶。

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凝血酶文献

An iridium oxide microelectrode for monitoring acute local pH changes of endothelial cells.

Analyst 140 , 4224-31, (2015)

pH sensors were fabricated by anodically electrodepositing iridium oxide films (AEIROFs) onto microelectrodes on chips and coated with poly(ethyleneimine) (PEI) for mechanical stability. These demonst...

Hinokitiol inhibits platelet activation ex vivo and thrombus formation in vivo

Biochem. Pharmacol. 85(10) , 1478-85, (2013)

Hinokitiol is a tropolone-related bioactive compound that has been used in hair tonics, cosmetics, and food as an antimicrobial agent. Recently, hinokitiol has attracted considerable interest because ...

Platelet activation and apoptosis modulate monocyte inflammatory responses in dengue.

J. Immunol. 193(4) , 1864-72, (2014)

Dengue is the most prevalent human arbovirus disease in the world. Dengue infection has a large spectrum of clinical manifestations, from self-limited febrile illness to severe syndromes accompanied b...

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凝血酶