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Magainin 2

Magainin 2用途

Magainin 2是在非洲爪蟾皮肤中发现的抗微生物多肽。

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Magainin 2名称

[ CAS 号 ]:
108433-95-0

[ 中文名 ]:
GLY-ILE-GLY-LYS-PHE-LEU-HIS-SER-ALA-LYS-LYS-PHE-GLY-LYS-ALA-PHE-VAL-GLY-GLU-ILE-MET-ASN-SER

[ 英文名 ]:
Magainin 2

[英文别名 ]:

Magainin I,10-L-lysine-22-L-asparagine
Mag-2
Magainin 2
magainin 2 peptide,Xenopus
Z-12 Peptide

Magainin 2生物活性

[ 描述 ]:

Magainin 2是在非洲爪蟾皮肤中发现的抗微生物多肽。

[ 相关类别 ]:

信号通路 >> 抗感染 >> 细菌

信号通路 >> 抗感染 >> 真菌

研究领域 >> 感染

天然产物 >> 其他

多肽产品

[体外研究]

Magainin 2在早期细胞凋亡中表现出杀菌作用并诱导大肠杆菌的形态学变化。 Magainin 2诱导细菌蛋白的表达,对caspase底物具有亲和力,并影响RecA作为半胱天冬酶样蛋白的表达[1]。 Magainin 2通过透化细胞膜杀死细菌,而对哺乳动物细胞没有显着的毒性。肽的主要靶标被认为是膜的脂质基质。将10μg/ mL magainin 2应用于池塘水中的原生动物草履虫(Paramecium caudatum)引起细胞的渗透性肿胀和随后的细胞破裂,表明该肽可能干扰负责渗透平衡的膜功能[2]。 Magainin 2以显着不同的方式渗透细菌和哺乳动物膜。该肽在巨大芽孢杆菌中形成直径约2.8nm(小于6.6nm)的孔,并易位到胞质溶胶中。相反,该肽显着扰乱CHO-K1细胞的膜,允许大分子(大于23nm)进入细胞质,伴随膜出芽和脂质触发,主要积聚在线粒体和细胞核中[3] ]。

[细胞实验]

将大肠杆菌细胞与magainin 2（50μg/ mL）一起温育。分别在温育0,2,4,6和8小时后获得培养物，并将其涂布在LB琼脂平板上，然后在37℃温育24小时后计数菌落形成单位。相对于对照治疗[1]确定存活百分比。

[参考文献]

[1]. Lee W, et al. Magainin 2 induces bacterial cell death showing apoptotic properties. Curr Microbiol. 2014 Dec;69(6):794-801.

[2]. Matsuzaki K, et al. Magainins as paradigm for the mode of action of pore forming polypeptides. Biochim Biophys Acta. 1998 Nov 10;1376(3):391-400.

[3]. Imura Y, et al. Magainin 2 in action: distinct modes of membrane permeabilization in living bacterial and mammalian cells. Biophys J. 2008 Dec 15;95(12):5757-65.

[相关活性小分子]

Magainin 2物理化学性质

[ 分子式 ]:
C₁₁₄H₁₈₀N₃₀O₂₉S

[ 分子量 ]:
2466.90000

[ 精确质量 ]:
2465.33000

[ PSA ]:
982.43000

[ LogP ]:
4.91300

[ 外观性状 ]:
固体

[ 储存条件 ]:
-20°C

Magainin 2MSDS

详细MSDS(安全技术说明书)

Magainin 2安全信息

[ 个人防护装备 ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ 危害码 (欧洲) ]:
T+

[ 危险品运输编码 ]:
NONH for all modes of transport

[ WGK德国 ]:
3

Magainin 2文献

Trypanosoma cruzi: synergistic cytotoxicity of multiple amphipathic anti-microbial peptides to T. cruzi and potential bacterial hosts.

Exp. Parasitol. 125(4) , 342-7, (2010)

The parasite Trypanasoma cruzi is responsible for Chagas disease and its triatomine vector, Rhodnius prolixus, has a symbiotic relationship with the soil bacterium, Rhodococcus rhodnii. R. rhodnii tha...

Design and characterization of novel hybrid antimicrobial peptides based on cecropin A, LL-37 and magainin II.

Peptides 33(2) , 197-205, (2012)

Antimicrobial peptides (AMPs) are a naturally occurring component of the innate immune response of many organisms and can have activity against both Gram-negative and Gram-positive bacterial species. ...

Magainins as paradigm for the mode of action of pore forming polypeptides.

Biochim. Biophys. Acta 1376 , 391-400, (1998)

Magainins are a class of antimicrobial peptides discovered in the skin of Xenopus laevis. The peptides kill bacteria by permeabilizing the cell membranes without exhibiting significant toxicity agains...

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