Aprotinin acetate salt structure
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Common Name | Aprotinin acetate salt | ||
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CAS Number | 9087-70-1 | Molecular Weight | 6511.83000 | |
Density | N/A | Boiling Point | N/A | |
Molecular Formula | C284H432N84O79S7 | Melting Point | N/A | |
MSDS | USA | Flash Point | N/A |
Use of Aprotinin acetate saltAprotinin is a bovine pancreatic trypsin inhibitor (BPTI) inhibitor which inhibits trypsin and chymotrypsin with Kis of 0.06 pM and 9 nM, respectively. |
Name | Aprotinin |
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Synonym | More Synonyms |
Description | Aprotinin is a bovine pancreatic trypsin inhibitor (BPTI) inhibitor which inhibits trypsin and chymotrypsin with Kis of 0.06 pM and 9 nM, respectively. |
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Related Catalog | |
Target |
Ki: 0.06 pM (Trypsin), 9 nM (Chymotrypsin)[1] |
In Vitro | Aprotinin, a serine protease inhibitor isolated from bovine lung, is a complex protease inhibitor that is an antifibrinolytic, inhibits contact activation, and decreases the inflammatory response to cardiopulmonary bypass[2]. Aprotinin inhibits trypsin (bovine, Ki= 0.06 pM), chymotrypsin (bovine, Ki= 9 nM), plasmin (human, 0.23 nM)[1]. Aprotinin is also a competitive protein inhibitor of NOS activity. It inhibits NOS-I and NOS-II with Ki values of 50 μM and 78 μM, respectively[3]. Aprotinin significantly inhibits fibrinolysis with an IC50 of 0.16±0.05 μM[4]. |
In Vivo | High dose aprotinin can reduce blood loss and transfusion requirements associated with primary cardiac procedures such as coronary artery bypass graft (CABG) or heart valve replacement surgery[5]. Aprotinin inhibits thrombus formation in a dose-dependent manner. Aprotinin at a dose of 1.5 mg kg-1 (bolus) and 3 mg kg-1 h-1 infusion (maintenance infusion) causes a tendency towards a reduction in bleeding time. Aprotinin significantly reduces the bleeding time starting at a dose of 3 mg kg-1 bolus plus 6 mg kg-1 h-1 showing a reduction of approximately 84%±2.9%. At the highest dose of 5 mg kg-1 and 10 mg kg-1 h-1, the strongest effects are observed[4]. Aprotinin may affect tumor necrosis factor-alpha (TNF) levels. Soluble TNFRI levels are significantly increased following I/R in the aprotinin treated wild type mice and not detected in all TNFRInull mice[6]. |
Animal Admin | Rats: Male Wistar rats (180-220 g) are used in the study. Aprotinin is dissolved in physiological saline. Aprotinin is administered by bolus injection followed by a maintenance infusion. The doses given are 1.5 mg kg-1 and 3 mg kg-1 h-1, 3mg kg-1 and 6 mg kg-1 h-1 up to 5 mg kg-1 and 10 mg kg-1 h-1. Plasma concentrations for the two agents are assessed by pharmacokinetic studies in rats[4]. Mice: An intact mouse model of ischemia/reperfusion (30 min-I/60 min-R) is used and left ventricular peak + dP/dt is measured in wild type mice (WT, C57BL/6; n=10), WT mice with aprotinin (4mL/kg; n=10), transgenic mice devoid of the TNFRI (TNFRInull; n=10), and TNFRInull with aprotinin (n=10)[6]. |
References |
Molecular Formula | C284H432N84O79S7 |
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Molecular Weight | 6511.83000 |
Exact Mass | 6507.00000 |
Appearance of Characters | lyophilized powder | white |
Storage condition | 2-8°C |
Stability | Stable. Incompatible with strong oxidizing agents. |
Water Solubility | glycerol: soluble3mg/mL, clear, colorless (equilibration buffer containing 5% glycerol) | Freely soluble in water and in aqueous buffers of low ionic strength. |
Personal Protective Equipment | Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter |
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Hazard Codes | Xn,Xi |
Risk Phrases | 42/43-36/37/38-20/21/22 |
Safety Phrases | 22-45-36/37-36-26 |
RIDADR | NONH for all modes of transport |
WGK Germany | 1 |
RTECS | YN5080000 |
HS Code | 35040000 |
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cas9004-04-0 |
Trypsin Inhibitor |
MFCD00130541 |