HOE 32020 is a Hoechst stain, which is a blue fluorescent dyes used to stain DNA.IC50 Value:These Bis-benzimides were originally developed by Hoechst AG, which numbered all their compounds so that the dye Hoechst 33342 is the 33342nd compound made by the company. There are three related Hoechst stains: Hoechst 33258, Hoechst 33342, and Hoechst 34580. The dyes Hoechst 33258 and Hoechst 33342 are the ones most commonly used and they have similarexcitation/emission spectra. Both dyes are excited by ultraviolet light at around 350 nm, and both emit blue/cyan fluorescent light around anemission maximum at 461 nm. Unbound dye has its maximum fluorescence emission in the 510-540 nm range. Hoechst dyes are soluble in water and in organic solvents such as dimethyl formamide or dimethyl sulfoxide. Concentrations can be achieved of up to 10 mg/mL. Aqueous solutions are stable at 2-6 °C for at least six months when protected from light. For long-term storage the solutions are instead frozen at ≤-20 °C.The dyes bind to the minor groove of double-stranded DNA with a preference for sequences rich in adenine andthymine. Although the dyes can bind to all nucleic acids, AT-rich double-stranded DNA strands enhance fluorescence considerably.Hoechst dyes are cell-permeable and can bind to DNA in live or fixed cells. Therefore, these stains are often called supravital, which means that cells survive a treatment with these compounds. Cells that express specific ATP-binding cassette transporter proteins can also actively transport these stains out of their cytoplasm.
1-Methoxynaphthalene is used as the substrate to investigate the activity of cytochrome c peroxidase (CcP). 1-Methoxynaphthalene also can be used to synthesize prenyl naphthalen-ols[1][2].
(+)-Norlirioferine is an alkaloid compound. (+)-Norlirioferine inhibits the cell growth of macrophages and VERO cells[1].
Glufosinate-d3 (hydrochloride) is a deuterium labeled compound.
Akuammiline, compound 2, is isolated from isolated from the seeds of Picralima klaineana[1].
Br-DAPI is a water-soluble, cell-permeable, DNA-binding photosensitizer.
Stigmastan-3β-ol-d5 is the deuterium labeled Stigmastan-3β-ol[1].
N3-D-Lys(Boc)-OH is a click chemistry reagent containing an Azide[1].
C6 NBD Ceramide is a Golgi apparatus fluorescent probe with cell membrane permeability. C6 NBD Ceramide can be used for fast and convenient green fluorescent labeling of Golgi in living and fixed cells, and can be used to observe changes in Golgi morphology in living cells (Ex=466 nm, Em=536 nm). C6-NBD-ceramide is metabolized to fluorescent sphingomyelin and glucosylceramide, can be used for the study of sphingolipid transport and metabolic mechanism[1][2][3].
Suc-Ala-Pro-Ala-AMC is a tripeptide substrate of elastase[1].
CFTR corrector 12 (compound 17C) is a bithiazole derivative, serving as CFTR corrector. CFTR corrector 12 has the ability to correct some folding defective mutants of the channel responsible for the control of chloride transport across the plasma membrane. CFTR corrector 12 recovers the α-sarcoglycan (α-SG) content in mutant cells[1].
(-)-β-Peltatin-5-O-beta-D-glucopyranoside is an active compound. (-)-β-Peltatin-5-O-beta-D-glucopyranoside can be isolated from P. peltatum L[1].
5-Methyl-2′-O-methylcytidine is a purine nucleoside analogue. Purine nucleoside analogs have broad antitumor activity targeting indolent lymphoid malignancies. Anticancer mechanisms in this process rely on inhibition of DNA synthesis, induction of apoptosis, etc[1].
MeOSuc-AAPF-CMK (MeOSuc-Ala-Ala-Pro-Phe-CH?Cl) is a potent proteinase K inhibitor[1].
A1AT modulator 1 is a potent inhibitor of Z ai-antitrypsin polymerisation with a pIC50 value of 8.3 [1].
Valinotricin is a fungal metabolite[1].
Guanosine 5'-triphosphate(GTP)-d4 (ammonium salt) is the deuterium labeled Guanosine 5'-triphosphate(GTP) ammonium salt[1].
Catechin 3-O-α-L-rhamnopyranoside is a natural product that can be isolated from the twigs of Artocarpus lakoocha[1].
DL-threo-Chloramphenicol D5 is a deuterium labeled DL-threo-Chloramphenicol. DL-threo-Chloramphenicol is the racemate of Chloramphenicol.
5-Carboxytetramethylrhodamine can be used as a fluorescent probe of nucleic acids and proteins. 5-Carboxytetramethylrhodamine displays excitation maxima of 558 nm and an emission maximum of 586 nm[1][2][3].
Myristoyl glutamic acid sodium is a common ingredient in cosmetics/creams. Myristoyl glutamic acid sodium, a surfactant belonging to the amino acid, has mild cleaning properties and is also foamy and mildly bacteriostatic[1].
Chrysoobtusin is an anthraquinone derivative isolated from Semen Cassiae. Semen Cassiae has long been used to protect liver, brighten eyes, and relieve constipation[1][2].
2-Methyl-3-buten-1-ol is a component of ylang-ylang oils extracted from the flowers[1].
Fmoc-Ser(tBu)-OH-15N is the 15N labeled Fmoc-Ser(tBu)-OH[1]. Fmoc-Ser(tBu)-OH is a serine derivative[2].
Fmoc-β-Ala-OH is an alanine derivative[1].
Dipsanoside A is a novel tetrairidoid glucoside from Dipsacus asper. Dipsacus asper Wall., a perennial plant widespread in China, has been used in traditional Chinese medicine for hundreds of years as a tonic for refreshment, as a fissiparism promoter of the osseous cells, and as an embryo security agent, etc[1].
H-Asp(Oet)-OEt.HCl is an aspartic acid derivative[1].
DiSC18(3) is a lipophilic carbocyanine dye for membrane labelling[1].
Dorzolamide(L671152; MK507) is an anti-glaucoma agent, which is a carbonic anhydrase inhibitor.Target: carbonic anhydrase (CA)Dorzolamide is a carbonic anhydrase inhibitor. It is an anti-glaucoma agent, and acts by decreasing the production of aqueous humour [1]. Glaucoma was induced in the right eye of adult Wistar rats by episcleral venous occlusion. One experimental group was administered dorzolamide 2%-timolol 0.5% combination eye drops, while the other experimental group was administered dorzolamide 2% eye drops. Control groups had surgery without drug administration. Drug application was initiated either 2 weeks before surgery (Group A), from the day of surgery (Group B), 2 weeks after surgery (Group C), or 4 weeks after surgery (Group D). RGCs were labeled by intratectal Fluorogold injections and counted from flat-mount preparations, and IOP was measured using Tonopen. Both dorzolamide-timolol combination and dorzolamide, when applied topically, significantly reduced IOP and improved RGC densities in experimental eyes when compared to control eyes. Earlier initiation, as well as longer duration of drug application, resulted in higher RGC densities [2].Clinical indications: Glaucoma; Ocular hypertensionFDA Approved Date: 1995Toxicity: Dizziness, headache, shortness of breath, slow heartbeat, severe asthma, cardiac arrest