Cathepsins are proteases (enzymes that degrades proteins) found in all animals as well as other organisms. The cathepsin family of proteolytic enzymes contains several diverse classes of proteases. Most of the members become activated at the low pH found in lysosomes. The activity of this family lies almost entirely within those organelles. Cathepsins have a vital role in mammalian cellular turnover, e.g. bone resorption. They degrade polypeptides and are distinguished by their substratespecificities. Classification: Cathepsin A, Cathepsin B, Cathepsin C, Cathepsin D, Cathepsin E, Cathepsin F, Cathepsin G, Cathepsin H, Cathepsin K, Cathepsin L1, Cathepsin L2, Cathepsin O, Cathepsin S, Cathepsin W, Cathepsin Z. Most cathepsins are lysosomal and each is involved in cellular metabolism, participating in various events such as peptide biosynthesis and protein degradation. Cathepsins may also cleave some protein precursors, thereby releasing regulatory peptides.


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Asperphenamate

Asperphenamate, a fungal metabolite of Aspergillus flatiipes with anti-cancer effect, exhibits IC50 values of 92.3 μM, 96.5 μM and 97.9 μM in T47D, MDA-MB-231 and HL-60 cells, respectively[1][2].

  • CAS Number: 63631-36-7
  • MF: C32H30N2O4
  • MW: 506.59200
  • Catalog: Autophagy
  • Density: 1.198g/cm3
  • Boiling Point: 774.3ºC at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 422.1ºC

4-Biphenylac-Cys(Me)-D-Arg-Phe-(2-phenylethyl)amide

Cathepsin L-IN-3 is a tripeptide-sized cathepsin L inhibitor.

  • CAS Number: 478164-48-6
  • MF: C41H49N7O4S
  • MW: 735.93700
  • Catalog: Cathepsin
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

H-Arg-Arg-AMC hydrochloride salt

Arg-Arg-AMC is a highly selective substrate of Cathepsin B. Arg-Arg-AMC can be used to cathepsin B activity assay in cancer cells, while cathepsin B is assocaited with cell invasive and metastatic phenotype in numerous types of cancer[1].

  • CAS Number: 263843-55-6
  • MF: C22H33N9O4
  • MW: 487.56
  • Catalog: Cathepsin
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

KGP-94

KGP-94 is a potent, selective cathepsin L inhibitor with IC50 of 189 nM, shows no significant inhibitory effect on cathepsin B (IC50>10 uM); significantly decreases the activity of cathepsin L toward human type I collagen, and impeds both migration and invasion of MDA-MB-231 human breast cancer cells; shows tumor growth retardation in a C3H mouse mammary carcinoma model.

  • CAS Number: 1131456-28-4
  • MF: C14H12BrN3OS
  • MW: 350.234
  • Catalog: Cathepsin
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

ONO-5334

A potent, selective, orally active inhibitor of cathepsin K with Ki of 0.1 nM, 0.049 nM and 0.85 nM for human, rabbit and rat cathepsin K, respectively; shows less or no potentcy for human cathepsin S/L/B/C (Ki=0.83/1.7/32/2500 nM); inhibits human osteoclasts bone resorption in vitro at a concentration more than 100 fold lower than that of alendronate; reduces plasma calcium level increased by PTHrP in thyroparathyroidectomized rats, decreases serum and urine C-telopeptide of type I collagen level. Osteoporosis Phase 2 Discontinued

  • CAS Number: 868273-90-9
  • MF: C21H34N4O4S
  • MW: 438.584
  • Catalog: Cathepsin
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

chymostatin

Chymostatin is a potent cathepsin G inhibitor. Chymostatin inhibits fungal growth when combined with other pepsin inhibitors. Chymostatin can be used for acute lung injury and pancreatitis research[1].

  • CAS Number: 9076-44-2
  • MF: C31H41N7O6
  • MW: 607.701
  • Catalog: Cathepsin
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: 276-278℃
  • Flash Point: N/A

Calpain-1 (pig)

Calpain-1 (pig) (μ-Calpain) is an intracellular Ca2+-regulated cysteine protease. Calpain-1 (pig) exhibits neuroprotective effect[1][2].

  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

MK-0674

MK-0674 is a potent, orally bioavailable and selective cathepsin K inhibitor, with an IC50 of 0.4 nM, shows 1156, 1465, 11857 and 243 fold selectivity over Cat B, Cat F, Cat L and Cat S. MK-0674 exhibits long half-lives in rats, rabbits and rhesus monkeys[1].

  • CAS Number: 887781-62-6
  • MF: C26H27F6N3O2
  • MW: 527.50200
  • Catalog: Cathepsin
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

3-Epiursolic acid

3-Epiursolic Acid is a triterpenoid isolated from Myrtaceae, acts as a competitive inhibitor of cathepsin L (ICIC50, 6.5 μM; Ki, 19.5 μM), with no obvious effect on cathepsin B[1].

  • CAS Number: 989-30-0
  • MF: C30H48O3
  • MW: 456.700
  • Catalog: Cathepsin
  • Density: 1.1±0.1 g/cm3
  • Boiling Point: 556.9±50.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 304.7±26.6 °C

Cathepsin L inhibitor III

Z-Phe-Tyr(tBu)-diazomethylketone is a potent cathepsin L inhibitor. Z-Phe-Tyr(tBu)-diazomethylketone mediates reovirus disassembly. Z-Phe-Tyr(tBu)-diazomethylketone decreases viral detection[1][2].

  • CAS Number: 114014-15-2
  • MF: C31H34N4O5
  • MW: 542.63
  • Catalog: SARS-CoV
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Cathepsin X-IN-1

Cathepsin X-IN-1 (compound 25) is a potent Cathepsin X inhibitor with an IC50 of 7.13 µM. Cathepsin X-IN-1 decreases PC-3 cell migration with low cytotoxic[1].

  • CAS Number: 2418577-51-0
  • MF: C15H13N3O3S
  • MW: 315.35
  • Catalog: Cathepsin
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

NA-CBZ-ARG-ARG 4-METHOXY-B-NAPHTHYLAMIDE

Z-Arg-Arg-4MβNA triacetate is a cathepsin B-specific substrate and can produce fluorescent end product 4MβNA (λex = 355 nm, λem = 430 nm)[1][2].

  • CAS Number: 100900-19-4
  • MF: C33H45N9O7
  • MW: 679.76600
  • Catalog: Cathepsin
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

E-64

E-64 is a potent irreversible inhibitor against general cysteine proteases with IC50 of 9 nM for papain.

  • CAS Number: 66701-25-5
  • MF: C15H27N5O5
  • MW: 357.405
  • Catalog: Autophagy
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: 182ºC
  • Flash Point: N/A

LHVS

LHVS is a potent, non-selective cysteine protease inhibitor[1]. LHVS effectively blocks T. gondii microneme protein secretion (IC50=10 μM), gliding motility, and cell invasion[2].

  • CAS Number: 170111-28-1
  • MF: C28H37N3O5S
  • MW: 527.68
  • Catalog: Parasite
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

H-Pro-Thr-Glu-Phe-p-nitro-Phe-Arg-Leu-OH

H-Pro-Thr-Glu-Phe-p-nitro-Phe-Arg-Leu-OH is a water-soluble polypeptide that can serve as a substrate for cathepsin D, pepsin and pepsinogen. H-Pro-Thr-Glu-Phe-p-nitro-Phe-Arg-Leu-OH has potential applications in biochemical analysis[1][2].

  • CAS Number: 90331-82-1
  • MF: C44H63N11O13
  • MW: 954.04
  • Catalog: Cathepsin
  • Density: 1.45g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

ALLM

ALLM (Calpain inhibitor II) is a potent inhibitor of calpain and cathepsin proteases. ALLM inhibits neuronal cell death and improves chronic neurological function after spinal cord injury (SCI)[1][2].

  • CAS Number: 110115-07-6
  • MF: C19H35N3O4S
  • MW: 401.564
  • Catalog: Cathepsin
  • Density: 1.1±0.1 g/cm3
  • Boiling Point: 676.5±55.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 362.9±31.5 °C

Gü2602

Gü2602 is a potent, reversible cathepsin K (CatK) inhibitor with a Ki of 0.013 nM for mature CatK (mCatK). Gü2602 suppresses the autocatalytic activation of the cathepsin K zymogen[1].

  • CAS Number: 1627094-88-5
  • MF: C16H22N4O3
  • MW: 318.37
  • Catalog: Cathepsin
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Z-Phe-Ala-diazomethylketone

Z-Phe-Ala-diazomethylketone binds directly to Aβ42 monomers and small oligomers. Z-Phe-Ala-diazomethylketone inhibits the formation of Aβ42 dodecamers and inhibits Aβ42 fibril formation in the solution. Z-Phe-Ala-diazomethylketone has the potential for neurodegenerative disorders research[1].

  • CAS Number: 71732-53-1
  • MF: C21H22N4O4
  • MW: 394.42400
  • Catalog: Cathepsin
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Cathepsin K inhibitor 5

Cathepsin K inhibitor 5 is a potent Cathepsin K inhibitor[1].

  • CAS Number: 448945-62-8
  • MF: C35H51N7O8
  • MW: 697.82
  • Catalog: Cathepsin
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

N-ALPHA-CBZ-ARG-ARG 7-AMIDO-4-METHYLCOUMARIN HYDROCHLORIDE

Z-Arg-Arg-AMC hydrochloride is a selective substrate of cathepsin B[1].

  • CAS Number: 136132-67-7
  • MF: C30H40ClN9O6
  • MW: 658.14800
  • Catalog: Cathepsin
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Z-Asp-Glu-Val-Asp-chloromethylketone

Z-DEVD-CMK is an irreversible inhibitor of most of the cathepsins in vitro[1].

  • CAS Number: 250584-13-5
  • MF: C27H35ClN4O12
  • MW: 643.04
  • Catalog: Cathepsin
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Z-FG-NHO-BzOME

Z-FG-NHO-BzOME is a cysteine protease inhibitor that selectively inhibits cathepsin B, cathepsin L, cathepsin S, and papain[1].

  • CAS Number: 180313-86-4
  • MF: C27H27N3O7
  • MW: 505.52
  • Catalog: Cathepsin
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

RKLLW-NH2

RKLLW-NH2 is a Cathepsin L inhibitor[1].

  • CAS Number: 289480-71-3
  • MF: C35H59N11O5
  • MW: 713.91
  • Catalog: Cathepsin
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

SSAA09E1

SSAA09E1 is a cathepsin L blocker (IC50: 5.33 μM).SSAA09E1 inhibits stages of viral entry. SSAA09E1 can be used for SARS-CoV infection research[1]

  • CAS Number: 433212-75-0
  • MF: C7H9N3S2
  • MW: 199.296
  • Catalog: SARS-CoV
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: 333.0±34.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 155.2±25.7 °C

Z-FA-FMK

(S,S)-Z-FA-FMK is a cell-permeable, irreversible cathepsin B inhibitor. (S,S)-Z-FA-FMK blocks LPS-induced production of IL-1α and IL-1β. (S,S)-Z-FA-FMK can be used as a negative control for caspase-1 and caspase-2 inhibitors because it lacks an aspartic acid residue at the P1 position[1][2].

  • CAS Number: 105637-38-5
  • MF: C21H23FN2O4
  • MW: 386.41700
  • Catalog: Cathepsin
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Z-LVG-CHN2

Z-LVG-CHN2 is a cell-permeable and irreversible inhibitor of cysteine proteinase. Z-LVG-CHN2 is a tripeptide derivative and mimics part of the human cysteine proteinase-binding center. Z-LVG-CHN2 displays an inhibition on HSV whereas no significant effect on poliovirus replication. Z-LVG-CHN2 effectively blocks SARS-COV-2 replication (EC50=190 nM) via inhibition of SARS-COV-2 3CL pro protease[3].

  • CAS Number: 119670-30-3
  • MF: C22H31N5O5
  • MW: 445.51
  • Catalog: HSV
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

2-Cyanopyrimidine

2-Cyanopyrimidine is a potent and non-selective cysteine protease cathepsin K inhibitor with an IC50 of 170 nM. 2-Cyanopyrimidine is used for osteoporos[1].

  • CAS Number: 14080-23-0
  • MF: C5H3N3
  • MW: 105.097
  • Catalog: Cathepsin
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 250.7±23.0 °C at 760 mmHg
  • Melting Point: 40-44 °C(lit.)
  • Flash Point: 97.2±7.8 °C

Ac-Leu-Val-Lys-aldehyde

Ac-Leu-Val-Lys-Aldehyde is a potent cathepsin B inhibitor with IC50s of 4 nM. Ac-Leu-Val-Lys-Aldehyde significantly reduces quinolinic acid (HY-100807)-induced striatal cell death and causes accumulation of LC3-II[1].

  • CAS Number: 147600-40-6
  • MF: C19H36N4O4
  • MW: 384.51
  • Catalog: Cathepsin
  • Density: 1.1±0.1 g/cm3
  • Boiling Point: 665.5±55.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 356.3±31.5 °C

Z-Phe-Phe-FMK

Cathepsin L-IN-2 (Z-Phe-Phe-FMK) is a potent and irreversible cathepsin L and cathepsin B inhibitor[1][2].

  • CAS Number: 108005-94-3
  • MF: C27H27FN2O4
  • MW: 462.51
  • Catalog: Cathepsin
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Aurantiamide acetate

Aurantiamide acetate (TMC-58A) is a selective and orally active cathepsin inhibitor isolated from Portulaca oleracea L. Aurantiamide acetate has anti-inflammatory activities and can be used for the study of  inflammatory diseases[1][2].

  • CAS Number: 56121-42-7
  • MF: C27H28N2O4
  • MW: 444.522
  • Catalog: Cathepsin
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 716.1±60.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 386.9±32.9 °C