HCV NS3-4A serine protease is a non-covalent heterodimer consisting of a catalytic subunit (the N-terminal one-third of NS3 protein) and an activating cofactor (NS4A protein), and is responsible for cleavage at four sites of the HCV polyprotein. HCV NS3-4A protease is essential for viral replication in cell culture and in chimpanzees, and has been considered as one of the most attractive targets for developing novel anti-HCV therapies. However, discovery of small-molecule, selective inhibitors against HCV NS3-4A protease as oral drug candidates has been hampered by its shallow substrate-binding groove and the lack of robust, reproducible viral replication models in cell culture or in small animals.


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HCV-IN-4

HCV-IN-4 is a potent and orally active HCV NS5A inhibitor, shows great potency against GT1a, GT2b, GT3a, GT1a Y93H and GT1a L31V, with EC90s of 3 pM, 0.3 nM, 0.01 nM, 0.5 nM and 0.02 nM, respectively[1].

  • CAS Number: 2058080-25-2
  • MF: C52H58FN9O8
  • MW: 956.07
  • Catalog: HCV
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Ledipasvir D-tartrate

Ledipasvir D-tartrate is an inhibitor of the hepatitis C virus NS5A, with EC50 values of 34 pM against GT1a and 4 pM against GT1b replicon.

  • CAS Number: 1502654-87-6
  • MF: C53H60F2N8O12
  • MW: 1039.09000
  • Catalog: HCV
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

GS-9256

GS-9256 is a selective HCV NS3 protease inhibitor. GS-9256 has good pharmacokinetic properties and antiviral activity[1].

  • CAS Number: 1001094-46-7
  • MF: C46H56ClF2N6O8PS
  • MW: 957.46100
  • Catalog: HCV Protease
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

AZD-7295

AZD-7295 is a HCV NS5A protein inhibitor, with an EC50 of 7 nM for GT-1b replicon[1][2].

  • CAS Number: 929890-64-2
  • MF: C32H35F3N4O5S
  • MW: 644.70
  • Catalog: HCV Protease
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Paritaprevir dihydrate

Paritaprevir (ABT-450) dihydrate is a potent, orally active and antiviral non-structural protein 3/4A (NS3/4A) protease inhibitor with EC50s of 1 and 0.21 nM against HCV 1a and 1b, respectively. Paritaprevir dihydrate is also a SARS-CoV 3CLpro inhibitor with an IC50 of 1.31 μM. Paritaprevir dihydrate is metabolized primarily by cytochrome P450 (CYP) 3A. The plasma concentration and half-life of Paritaprevir dihydrate can be enhanced by Ritonavir (a CYP450 inhibitor)[1][2][3][4].

  • CAS Number: 1456607-71-8
  • MF: C40H43N7O7S.2(H2O)
  • MW: 801.908
  • Catalog: HCV
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

R-7128

Mericitabine (R-7128) is a nucleoside inhibitor of the HCV NS5B polymerase that acts as an RNA chain terminator and prevents elongation of RNA transcripts during replication.

  • CAS Number: 940908-79-2
  • MF: C18H26FN3O6
  • MW: 399.41400
  • Catalog: HCV
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Isoeuphorbetin

Isoeuphorbetin, a dimeric coumarin isolated from Viola philippica, is a potent HCV protease inhibitor with an IC50 of 3.63 µg/mL[1].

  • CAS Number: 50886-61-8
  • MF: C18H10O8
  • MW: 354.26700
  • Catalog: HCV Protease
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

AL-611

AL-611 is an HCV NS5B polymerase inhibitor (EC50 = 5 nM).

  • CAS Number: 1613591-70-0
  • MF: C25H33F2N6O8P
  • MW: 614.54
  • Catalog: HCV Protease
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

NS5A-IN-4

NS5A-IN-4 (Compound 1.12) is an orally active pan-genotypic hepatitis C virus (HCV) NS5A inhibitor with IC50 values of 1.2, 2296, 4.6, 362, 10.3 and 693 pM against gT1b, gT1a, gT2a, gT3a, gT4a and gT5a[1].

  • CAS Number: 2088243-03-0
  • MF: C47H48N8O6
  • MW: 820.93
  • Catalog: HCV Protease
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

JTK-109

JTK-109 is an inhibitor of hepatitis C virus NS5B RNA-dependent RNA polymerase inhibitor and inhibits G1b and G3a subgenomic replicons and recombinant enzymes.

  • CAS Number: 480462-62-2
  • MF: C37H33ClFN3O4
  • MW: 638.13
  • Catalog: HCV Protease
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

PSI-6130

PSI-6130 is a potent and selective inhibitor of HCV NS5B polymerase, and inhibits HCV replication with a mean IC50 of 0.6 μM. 

  • CAS Number: 817204-33-4
  • MF: C10H14FN3O4
  • MW: 259.234
  • Catalog: HCV
  • Density: 1.7±0.1 g/cm3
  • Boiling Point: 482.4±55.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 245.5±31.5 °C

Ciluprevir

Ciluprevir(BILN 2061) is a specific and potent peptidomimetic inhibitor of the HCV NS3 protease with an IC50 of 3.0 nM.

  • CAS Number: 300832-84-2
  • MF: C40H50N6O8S
  • MW: 774.93
  • Catalog: HCV
  • Density: 1.4±0.0 g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

voxilaprevir

Voxilaprevir (GS-9857) is a fluorinated macrocyclic hepatitis C virus (HCV) nonstructural protein (NS) 3/4A protease inhibitor with potent in vitro antiviral activity against genotypes 1-6 HCV and broad coverage of NS3/4A protease polymorphisms. GS-9857 improves coverage against commonly encountered NS3 resistance-associated variants (RAVs)[1][2].

  • CAS Number: 1535212-07-7
  • MF: C40H52F4N6O9S
  • MW: 868.93400
  • Catalog: HCV Protease
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Coblopasvir

Coblopasvir (KW136, KW-136) is a novel HCV NS5A inhibitor under development for treatment of HCV infection. HCV Infection Phase 3 Clinical

  • CAS Number: 1312608-46-0
  • MF: C41H50N8O8
  • MW: 782.885
  • Catalog: HCV
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 1102.5±65.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 620.6±34.3 °C

Simeprevir

Simeprevir is a potent HCV NS3/4A protease inhibitor which suppresses HCV replication with EC50 of 8 nM.

  • CAS Number: 923604-59-5
  • MF: C38H47N5O7S2
  • MW: 749.939
  • Catalog: HCV
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

H-Glu-Ala-Gly-Asp-Asp-Ile-Val-Pro-Cys-Ser-Met-Ser-Tyr-Thr-Trp-Thr-Gly-Ala-OH

Hepatitis C Virus S5A/5B is a synthetic peptide substrate. Hepatitis C Virus S5A/5B mimics the NS5A/5B junction of the nonstructural protein (NS), served as the substrate for the study of HCV NS3 protease activity[1].

  • CAS Number: 191529-67-6
  • MF: C81H119N19O30S2
  • MW: 1903.051
  • Catalog: HCV Protease
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: 2224.5±65.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 1299.1±34.3 °C

Filibuvir

Filibuvir is a potent, selective non-nucleoside inhibitor (NNI) of the HCV nonstructural 5B protein (NS5B) RNA-dependent RNA polymerase, and it binds noncovalently in the “Thumb 2” pocket of NS5B. In vitro, filibuvir is equipotent against genotype 1a and 1b replicons, with an EC50 of 59 nM[1].

  • CAS Number: 877130-28-4
  • MF: C29H37N5O3
  • MW: 503.63600
  • Catalog: HCV Protease
  • Density: 1.29g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Danoprevir (ITMN-191)

Danoprevir is a NS3/4A protease inhibitor for hepatitis C virus (HCV) with IC50 of 0.2-3.5 nM. The inhibition effect on HCV genotypes 1A/1B/4/5/6 is approximately 10-fold higher than 2B/3A.

  • CAS Number: 850876-88-9
  • MF: C35H46FN5O9S
  • MW: 731.831
  • Catalog: HCV
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

BI-1230

BI-1230 is potent and orally active digit nanomolar inhibitor of HCV NS3 protease and of viral replication. BI-1230 is also highly selective against other serine/cysteine proteases. BI-1230 shows good Pharmacokinetic(PK) activity[1].

  • CAS Number: 849022-32-8
  • MF: C42H52N6O9S
  • MW: 816.96
  • Catalog: HCV Protease
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Elbasvir

Elbasvir (MK-8742) is a hepatitis C virus nonstructural protein 5A (HCV NS5A) inhibitor with EC50s of 4, 3 and 3 nM against genotype 1a, 1b, and 2a, respectively.

  • CAS Number: 1370468-36-2
  • MF: C49H55N9O7
  • MW: 882.017
  • Catalog: HCV
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Ac-Glu-Glu-Val-Val-Ala-Cys-pNA

Ac-EEVVAC-pNA is a chromogenic substrate for a continuous spectrophotometric assay of HCV NS3 protease. The sequence EEVVAC is derived from the 5A-5B cleavage junction of the HCV polyprotein[1].

  • CAS Number: 389868-12-6
  • MF: C34H50N8O13S
  • MW: 810.872
  • Catalog: HCV Protease
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 1289.9±65.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 733.9±34.3 °C

VX-222 (VCH-222, Lomibuvir)

VX-222 (VCH-222) is a novel, potent and selective inhibitor of HCV polymerase with IC50 of 0.94-1.2 μM, 15.3-fold less effective for mutant M423T, and 108-fold less effective for mutant I482L.IC50 Value: 0.94 μM (HCV NS5B 1a); 1.2 μM (HCV NS5B 1b)Target: HCVVX-222 is a small molecule non-nucleoside inhibitor of HCV NS5B polymerase that is being investigated for the treatment of hepatitis C virus infection. VX-222 exhibits non-competitive and selective inhibition in HCV NS5B of genotype 1a and 1b, with IC50 of 0.94 and 1.2 μM, respectively. VX-222 selectively inhibits the replication of subgenomic HCV genotype 1a and 1b with an EC50 of 22.3 and 11.2 nM, respectively. [1] Similarly, a recent study shows that VX-222 inhibits the 1b/Con1 HCV subgenomic replicon, with an EC50 of 5 nM. In rats and dogs, VCH-222 displays fine pharmacokinetic pro le, including low total body clearance and excellent oral bioavailability (greater than 30%) and good ADME properties. VCH-222 is biotransformed by several enzymes (CYP1A1, 2A6, 2B6, 2C8, CYP 3A4, UGT1A3) and is predicted to be actively transported in liver and excreted mainly intact in bile or as glucuronide adducts.

  • CAS Number: 1026785-59-0
  • MF: C25H35NO4S
  • MW: 445.615
  • Catalog: HCV
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 640.5±55.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 341.2±31.5 °C

Coblopasvir dihydrochloride

Coblopasvir (KW-136) dihydrochloride is a pangenotypic non-structural protein 5A (NS5A) inhibitor. Coblopasvir dihydrochloride can be used for research of chronic hepatitis C virus infection[1].

  • CAS Number: 1966138-53-3
  • MF: C41H52Cl2N8O8
  • MW: 855.81
  • Catalog: HCV
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Faldaprevir

Faldaprevir (BI 201335) is a potent, orally active and selective noncovalent inhibitor of NS3/4A protease of HCV (hepatitis C virus) genotypes 1a and 1b, with Ki values of 2.6 and 2.0 nM, respectively. Faldaprevir inhibits HCV RNA replication, with EC50 values of 6.5 and 3.1 nM, respectively. Faldaprevir has potent antiviral activity against chronic HCV infection[1][2].

  • CAS Number: 801283-95-4
  • MF: C40H49BrN6O9S
  • MW: 869.82
  • Catalog: HCV Protease
  • Density: 1.5±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

BI 653048 phosphate

An oral glucocorticoid agonist for the treatment of rheumatoid arthritis. Rheumatoid Arthritis Phase 1 Clinical

  • CAS Number: 1198784-97-2
  • MF: C23H28F4N3O8PS
  • MW: 613.517
  • Catalog: Glucocorticoid Receptor
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Grazoprevir sodium salt

Grazoprevir sodium salt (MK-5172 sodium salt) is a selective inhibitor of Hepatitis C virus NS3/4a protease with broad activity across genotypes and resistant variants, with Kis of 0.01 nM (gt1b), 0.01 nM (gt1a), 0.08 nM (gt2a), 0.15 nM (gt2b), 0.90 nM (gt3a), respectively.

  • CAS Number: 1425038-27-2
  • MF: C38H49N6NaO9S
  • MW: 788.885
  • Catalog: HCV
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

ACH-806

ACH-806 is an NS4A antagonist which can inhibit Hepatitis C Virus (HCV) replication with an EC50 of 14 nM.

  • CAS Number: 870142-71-5
  • MF: C19H20F3N3O2S
  • MW: 411.44100
  • Catalog: HCV
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

IDX184

IDX184 is a potent and orally bioavailable inhibitor of HCV replication. IDX184 potently inhibits HCV polymerase (IC50=0.31 μM, Ki=52.3 nM)[1][2].

  • CAS Number: 1036915-08-8
  • MF: C25H35N6O9PS
  • MW: 626.61900
  • Catalog: HCV
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Ledipasvir diacetone

Ledipasvir diacetone is the active pharmaceutical ingredient of Ledipasvir. Ledipasvir is an inhibitor of the hepatitis C virus NS5A, with EC50 values of 34 pM against GT1a and 4 pM against GT1b replicon.

  • CAS Number: 1502655-48-2
  • MF: C55H66F2N8O8
  • MW: 1005.16000
  • Catalog: HCV
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A