Acetylcholinesterase (AChE or acetylhydrolase) is a hydrolase that hydrolyzes the neurotransmitter acetylcholine. AChE is found at mainly neuromuscular junctions and cholinergic brain synapses, where its activity serves to terminate synaptic transmission. It belongs tocarboxylesterase family of enzymes. It is the primary target of inhibition by organophosphorus compounds such as nerve agents and pesticides. AChE has a very high catalytic activity - each molecule of AChE degrades about 25000 molecules ofacetylcholine (ACh) per second, approaching the limit allowed by diffusion of the substrate. ACh is released from the nerve into the synaptic cleft and binds to ACh receptors on the post-synaptic membrane, relaying the signal from the nerve. AChE, also located on the post-synaptic membrane, terminates the signal transmission by hydrolyzing ACh. The liberated choline is taken up again by the pre-synaptic nerve and ACh is synthetized by combining with acetyl-CoA through the action of choline acetyltransferase.


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(+)-PHENSERINE

(+)-Phenserine is a novel selective cholinesterase noncompetitive inhibitor with an IC50 of 45.3 μM.

  • CAS Number: 116839-68-0
  • MF: C20H23N3O2
  • MW: 337.41600
  • Catalog: AChE
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Pirimiphos-methyl-d6

Pirimiphos-methyl-d6 is the deuterium labeled Pirimiphos-methyl. Pirimiphos-methyl is a rapid-acting organophosphorus insecticide and acaricide, causing inhibition of AChE in target organisms. Pirimiphos-methyl is often used for prevention and control of beetles, snout beetles, moths and Ephestia cautella during storage of agricultural grains[1][2][3].

  • CAS Number: 1793055-06-7
  • MF: C11H14D6N3O3PS
  • MW: 311.371
  • Catalog: AChE
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 386.5±52.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 187.6±30.7 °C

Zanapezil

Zanapezil (TAK-147) fumarate is a potent, reversible and selective acetylcholine esterase (AChE) inhibitor. Zanapezil fumarate shows a potent and reversible inhibition of AChE activity in homogenates of the rat cerebral cortex (IC50=51.2 nM). Zanapezil fumarate shows a moderate inhibition of muscarinic M1 and M2 receptor binding with Ki values of 234 and 340 nM, respectively. Zanapezil fumarate can be used for the research of early stages of Alzheimer's disease (AD)[1][2].

  • CAS Number: 263248-42-6
  • MF: C29H36N2O5.xC4H4O4
  • MW: 492.60700
  • Catalog: AChE
  • Density: N/A
  • Boiling Point: 547ºC at 760mmHg
  • Melting Point: N/A
  • Flash Point: 284.6ºC

(S)-Donepezil

(S)-Donepezil is a S-enantiomer of Donepezil (HY-14566). Donepezil is a specific and potent AChE inhibitor[1][2].

  • CAS Number: 142057-80-5
  • MF: C24H29NO3
  • MW: 379.49
  • Catalog: AChE
  • Density: 1.1±0.1 g/cm3
  • Boiling Point: 527.9±50.0 °C at 760 mmHg
  • Melting Point: 206.72° C
  • Flash Point: 273.1±30.1 °C

AChE/BChE-IN-4

AChE/BChE-IN-4 (BMC-3) is a dual AChE and BChE inhibitor with IC50 values of 792 nM and 2.2 nM against human AChE (hAChE) and human BChE (hBChE), respectively. AChE/BChE-IN-4 can cross the BBB[1].

  • CAS Number: 2294963-47-4
  • MF: C17H26N2O3
  • MW: 306.40
  • Catalog: AChE
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

2-[3-(4-Hydroxyphenyl)propyl]-5-methoxyphenol

Broussonin A is a potent BChE inhibitor, with an IC50 of 4.16 µM. Broussonin A is a diarylpropane natural product that can be isolated from the bark of Broussonetia papyrifera after solid fermentation[1][2][3].

  • CAS Number: 73731-87-0
  • MF: C16H18O3
  • MW: 258.312
  • Catalog: AChE
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 439.5±35.0 °C at 760 mmHg
  • Melting Point: 101-101.5 °C
  • Flash Point: 219.6±25.9 °C

Sophoflavescenol

Sophoflavescenol is a prenylated flavonol, which shows great inhibitory activity with IC50 of 0.013 μM against Phosphodiesterase 5 (PDE5), and also inhibits RLAR, HRAR, AGE, BACE1, AChE and BChE with IC50s of 0.30 µM, 0.17 µM, 17.89 µg/mL, 10.98 µM, 8.37 µM and 8.21 µM, respectively.

  • CAS Number: 216450-65-6
  • MF: C21H20O6
  • MW: 368.380
  • Catalog: Phosphodiesterase (PDE)
  • Density: 1.359±0.06 g/cm3
  • Boiling Point: 631.8±55.0 °C at 760 mmHg
  • Melting Point: 273-275 ºC
  • Flash Point: 226.2±25.0 °C

Nodakenin

Nodakenin is a major coumarin glucoside in the root of Peucedanum decursivum Maxim. Nodakenin inhibits acetylcholinesterase (AChE) activity with an IC50 of 84.7 μM[1][2].

  • CAS Number: 495-31-8
  • MF: C20H24O9
  • MW: 408.399
  • Catalog: AChE
  • Density: 1.5±0.1 g/cm3
  • Boiling Point: 635.4±55.0 °C at 760 mmHg
  • Melting Point: melting at 216°
  • Flash Point: 225.4±25.0 °C

Sinapine hydroxide

Sinapine hydroxide is an alkaloid isolated from seeds of the cruciferous species. Sinapine hydroxide exhibits anti-inflammatory, anti-oxidant, anti-tumor, anti-angiogenic and radio-protective effects. Sinapine hydroxide is also an acetylcholinesterase (AChE) inhibitor and can be used for the research of Alzheimer’s disease, ataxia, myasthenia gravis, and Parkinson’s disease[1][2][3][4].

  • CAS Number: 122-30-5
  • MF: C16H25NO6
  • MW: 327.37
  • Catalog: P-glycoprotein
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

8H-Benzo[g]-1,3-benzodioxolo[6,5,4-de]quinolin-8-one,10-methoxy

Lanuginosine (Compound 12) is an AChE inhibitor (IC50: 10.9 μM). Lanuginosine inhibits Aβ aggregation. Lanuginosine can be used for research of Alzheimer’s disease[1].

  • CAS Number: 23740-25-2
  • MF: C18H11NO4
  • MW: 305.28
  • Catalog: AChE
  • Density: 1.472g/cm3
  • Boiling Point: 572.5ºC at 760mmHg
  • Melting Point: N/A
  • Flash Point: 300.1ºC

Sclerone

(Rac)-Sclerone is a natural product, that can be isolated from the green husk of Carya illinoinensis. (Rac)-Sclerone shows appreciable inhibition of AChE, with an IC50 of 192.65 ± 10.12 μg/mL[1].

  • CAS Number: 19638-58-5
  • MF: C10H10O3
  • MW: 178.18
  • Catalog: AChE
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

AChE/HDAC-IN-1

COX-2-IN-23 (compound A10) is a potent both AChE and HDAC inhibitor with IC50 values of 0.12 and 0.23 nM. COX-2-IN-23 exhibits antioxidant activity and metal chelating properties. COX-2-IN-23 can be used in alzheimer's disease research[1].

  • CAS Number: 2414053-06-6
  • MF: C26H27ClN4O3
  • MW: 478.97
  • Catalog: HDAC
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Corydaline

Corydaline is an acetylcholinesterase inhibitor isolated from Corydalis yanhusuo.

  • CAS Number: 518-69-4
  • MF: C22H27NO4
  • MW: 369.454
  • Catalog: AChE
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 482.3±45.0 °C at 760 mmHg
  • Melting Point: 135°
  • Flash Point: 135.7±25.9 °C

Virgaureagenin G

Polygalacic acid, is a triterpene, isolated from the root of Polygala tenuifolia Willd. Polygalacic acid inhibits MMP expression. Polygalacic acid may have a therapeutic effect in Osteoarthritis (OA) treatment [1]. Polygalacic acid exerts a significant neuroprotective effect on cognitive impairment, PA improves cholinergic system reactivity by inhibiting acetylcholinesterase (AChE) activity, increasing choline acetyltransferase (ChAT) activity, and elevating levels of acetylcholine (Ach) in the hippocampus and frontal cortex[2].

  • CAS Number: 22338-71-2
  • MF: C30H48O6
  • MW: 504.698
  • Catalog: MMP
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 638.1±55.0 °C at 760 mmHg
  • Melting Point: 315-318ºC
  • Flash Point: 353.7±28.0 °C

Rhodiosin

Rhodiosin, isolated from the root of Rhodiola crenulata, is a specific non-competitive cytochrome P450 2D6 inhibitor with an IC50 of 0.420 μM and a Ki of 0.535 μM[1]. Rhodiosin exhibits potent, dose-dependent inhibitory effects on acetylcholinesterase (AChE) with IC50 ranged from 57.50 to 2.43 μg/mL[2]. Rhodiosin exhibits potent DPPH free radical scavenging activities, with an IC50 of 27.77 μM[3].

  • CAS Number: 86831-54-1
  • MF: C27H30O16
  • MW: 610.518
  • Catalog: AChE
  • Density: 1.8±0.1 g/cm3
  • Boiling Point: 994.3±65.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 329.1±27.8 °C

Axillaridine A

Axillaridine A can be isolated from Sarcococca hookeriana var. digyna. Axillaridine A inhibits the catalytic activity of AChE[1].

  • CAS Number: 128255-16-3
  • MF: C30H42N2O2
  • MW: 462.667
  • Catalog: AChE
  • Density: 1.1±0.1 g/cm3
  • Boiling Point: 611.4±47.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 323.6±29.3 °C

α-Glycosidase-IN-1

α-Glycosidase-IN-1 (compound MZ7) is a potent α-GLY (α-Glycosidase) inhibitor, with an IC50 of 44.72 nM and a KI of 41.74 nM. α-Glycosidase-IN-1 also shows inhibition profile against human carbonic anhydrase isoenzymes I and II (hCA I and hCA II), and acetylcholinesterase (AChE), with IC50 values of 104.87, 100.04, and 654.87 nM, respectively. α-Glycosidase-IN-1 can be used for the research of many diseases such as diabetes, Alzheimer’s disease, heart failure, ulcer, and epilepsy[1].

  • CAS Number: 2428389-66-4
  • MF: C21H19N9O6S2
  • MW: 557.56
  • Catalog: Carbonic Anhydrase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

AChE/BuChE-IN-3

AChE/BuChE-IN-3 (compound C4) is a potent and blood-brain barrier (BBB) penetrant AChE and BuChE dual inhibitor with IC50s of 0.65 μM and 5.77 μM for AChE and BuChE. AChE/BuChE-IN-3 also inhibits Aβ1-42 aggregation. AChE/BuChE-IN-3 has effectively neuroprotective activities and nearly no toxicity on SH-SY5Y cells. AChE/BuChE-IN-3 can be used for researching Alzheimer's disease[1].

  • CAS Number: 2742707-47-5
  • MF: C30H30F3N3O6
  • MW: 585.57
  • Catalog: AChE
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Physostigmine salicylate

Physostigmine salicylate (Eserine salicylate) is a reversible acetylcholinesterase (AChE) inhibitor. Physostigmine salicylate crosses the blood-brain barrier and stimulates central cholinergic neurotransmission. Physostigmine salicylate can reverse memory deficits in transgenic mice with Alzheimer's disease. Physostigmine salicylate is also an antidote for anticholinergic poisoning[1][2][3][4].

  • CAS Number: 57-64-7
  • MF: C22H27N3O5
  • MW: 413.46700
  • Catalog: AChE
  • Density: N/A
  • Boiling Point: 393.5ºC at 760 mmHg
  • Melting Point: 181-183 °C
  • Flash Point: 191.8ºC

Echothiophate Iodide

Echothiopate iodide is a potent hBChE (human butyrylcholinesterase) inhibitor. Echothiopate iodide is a long-acting anticholinesterase agent. Echothiopate iodide can be used for glaucoma research[1][2].

  • CAS Number: 513-10-0
  • MF: C9H23INO3PS
  • MW: 383.22700
  • Catalog: AChE
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Native Electrophorus electricus (electric eel) Acetylcholinesterase

Acetylcholinesterase (ACHE; EC 3.1.1.7) is a cholinergic enzyme mainly found in neuromuscular junctions and cholinergic type chemical synapses, and is often used in biochemical research. Acetylcholinesterase catalyzes the breakdown or hydrolysis of acetylcholine and some other choline esters that act as neurotransmitters into acetate and choline. Acetylcholinesterase's main role is to terminate neuronal transmission and signaling between synapses to prevent ACh spread and activation of nearby receptors[1].

  • CAS Number: 9000-81-1
  • MF: C31H27NO4
  • MW: 477.550
  • Catalog: AChE
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 643.1±55.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 342.7±31.5 °C

(+)-Pteryxin

Pteryxin, a coumarin in Peucedanum japonicum Thunb leaves, exerts antiobesity activity[1]. Pteryxin is a potent butyrylcholinesterase (BChE) inhibitor, with an IC50 of 12.96 μg/ml[2].

  • CAS Number: 13161-75-6
  • MF: C21H22O7
  • MW: 386.395
  • Catalog: AChE
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 486.8±45.0 °C at 760 mmHg
  • Melting Point: 81℃
  • Flash Point: 211.5±28.8 °C

Ladostigil

Ladostigil (TV-3326) is a dual inhibitor of cholinesterase and brain-selective monoamine oxidase (MAO), with an IC50 of 37.1 and 31.8 μM for MAO-B and AChE, reapectively. Ladostigil could increase cholinergic transmission, prevent the formation of ROS or their actions and be used for the research of depression and Alzheimer's disease[1][2].

  • CAS Number: 209394-27-4
  • MF: C16H20N2O2
  • MW: 272.34200
  • Catalog: AChE
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

hCAI/II-IN-5

hCAI/II-IN-5 (compound MZ8) is a potent hCA I and hCA II (human carbonic anhydrase isoenzymes I and II) inhibitor, with IC50 values of 37.88 and 45.23 nM, respectively. hCAI/II-IN-5 also shows inhibition profile against α-Glycosidase and AChE, with IC50 values of 48.98 and 420.14 nM, respectively. hCAI/II-IN-5 can be used for the research of many diseases such as diabetes, Alzheimer’s disease, heart failure, ulcer, and epilepsy[1].

  • CAS Number: 2428389-67-5
  • MF: C21H18Cl2N8O4S2
  • MW: 581.45
  • Catalog: Carbonic Anhydrase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Tacrine hydrochloride

Tacrine hydrochloride is a potent inhibitor of both AChE and BChE, with IC50s of 31 nM and 25.6 nM, respectively. Tacrine hydrochloride is also a NMDAR inhibitor, with an IC50 of 26 μM. Tacrine hydrochloride can be used for the research of Alzheimer’s disease[1][2].

  • CAS Number: 1684-40-8
  • MF: C13H15ClN2
  • MW: 270.755
  • Catalog: iGluR
  • Density: N/A
  • Boiling Point: 409.4ºC at 760mmHg
  • Melting Point: 280-284 °C(lit.)
  • Flash Point: 230.5ºC

Swertianolin

Swertianolin, a xanthone isolated from Gentianella Acuta, inhibits acetylcholinesterase (AChE). Swertianolin also exhibits anti-HBV and anti-bacterial activity[1][2].

  • CAS Number: 23445-00-3
  • MF: C20H20O11
  • MW: 436.366
  • Catalog: Bacterial
  • Density: 1.7±0.1 g/cm3
  • Boiling Point: 806.3±65.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 287.7±27.8 °C

Pirimicarb

Pirimicarb is a fast-acting selective carbamate insecticide on a wide range of crops including cereals, sugar beet, potatoes, fruits and vegetables. Pirimicarb is an AChE inhibitor and an acaricide[1].

  • CAS Number: 23103-98-2
  • MF: C11H18N4O2
  • MW: 238.286
  • Catalog: AChE
  • Density: 1.1±0.1 g/cm3
  • Boiling Point: 373.4±52.0 °C at 760 mmHg
  • Melting Point: 90.5°C
  • Flash Point: 179.6±30.7 °C

AChE-IN-20

AChE-IN-20 (compound M26) is a potent AChE inhibitor with IC50 value of 397.32 nM and with Ki value of 335.76 nM. AChE-IN-20 shows inhibition potency against hCA I , hCA II and α-GLY with IC50 values of 84.14, 69.24 and 52.08 nM and with Ki values of 97.86, 76.23 and 57.93 nM, respectively[1].

  • CAS Number: 2428389-65-3
  • MF: C22H20N8O6S2
  • MW: 556.57
  • Catalog: AChE
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

MHP 133

HY-101653 is a drug with multiple CNS targets, and inhibits acetylcholinesterase (AChE) with Ki of 69 μM; also active against muscarinic M1 and M2 receptors, serotonin 5HT4 receptors, and imidazole I2 receptors.

  • CAS Number: 147340-43-0
  • MF: C17H20ClN5O3
  • MW: 377.825
  • Catalog: 5-HT Receptor
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

3β-Hydroxy-lup-20(29)-en-16-one

3β-Hydroxy-lup-20(29)-en-16-one (compound 9) is a dual inhibitor of AChE and BChE, with selectivity for BChE (IC50: 28.9 μM)[1].

  • CAS Number: 65043-60-9
  • MF: C30H48O2
  • MW: 440.70
  • Catalog: AChE
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A