ROCK (Rho-associated protein kinase) is a kinase belonging to the AGC (PKA/ PKG/PKC) family of serine-threonine kinases. ROCKs (ROCK1 and ROCK2) occur in mammals, zebrafish, Xenopus, invertebrates and chicken. Human ROCK1 has a molecular mass of 158 kDa and is a major downstream effector of the small GTPase RhoA. Mammalian ROCK consists of a kinase domain, acoiled-coil region and a Pleckstrin homology (PH) domain, which reduces the kinase activity of ROCKs by an autoinhibitory intramolecular fold if RhoA-GTP is not present. ROCK plays a role in a wide range of different cellular phenomena, as ROCK is a downstream effector protein of the small GTPase Rho, which is one of the major regulators of the cytoskeleton.


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AR-13324 (hydrochloride)

Netarsudil hydrochloride is a ROCK, and norepinephrine transporter inhibitor. Target: ROCKin vitro: AR-13324 is a small-molecule inhibitor of Rho kinase and a norepinephrine transporter; reduces intraocular pressure (IOP) in normotensive monkey eyes.[1] AR-13324 is a small-molecule inhibitor of Rho kinase and a norepinephrine transporter.[2]in vivo: AR-13324 produces statistically significant lowering of episcleral venous pressure (EVP) in Dutch Belted (DB) rabbits. [3]

  • CAS Number: 1253952-02-1
  • MF: C28H29Cl2N3O3
  • MW: 526.454
  • Catalog: ROCK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Gsk270822a

GSK270822A is a selective ROCK1 inhibitor. GSK270822A exhibits IC50 of 9nM, 1100nM, 1550nM for ROCK1, RSK1, p70S6K, respectively.

  • CAS Number: 864082-23-5
  • MF: C24H20N4O2
  • MW: 396.44
  • Catalog: ROCK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

TC-S 7001

Azaindole 1 is a highly potent inhibitor of human ROCK-1 and ROCK-2, with IC50s of 0.6 and 1.1 nM, respectively, and also inhibits murine ROCK-2 or rat ROCK-2 with IC50s of 2.4 and 0.8 nM, respectively.

  • CAS Number: 867017-68-3
  • MF: C18H13ClF2N6O
  • MW: 402.78500
  • Catalog: ROCK
  • Density: 1.61
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

GSK429286A

GSK429286A is a selective inhibitor of ROCK1 with an IC50 value of 14 nM.

  • CAS Number: 864082-47-3
  • MF: C21H16F4N4O2
  • MW: 432.371
  • Catalog: ROCK
  • Density: 1.5±0.1 g/cm3
  • Boiling Point: 688.4±55.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 370.2±31.5 °C

SB 772077B dihydrochloride

SB-772077B dihydrochloride is an aminofurazan-based Rho kinase( ROCK) inhibitor with IC50s of 5.6 nM and 6 nM toward ROCK1 and ROCK2, respevtively[1].

  • CAS Number: 607373-46-6
  • MF: C15H20Cl2N8O2
  • MW: 415.28
  • Catalog: ROCK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Scaff10-8

Scaff10-8 is an inhibitor of AKAP-Lbc-RhoA interaction that acts by promoting the translocation of aquaporin-2 to the plasma membrane of renal collecting duct principal cells.

  • CAS Number: 777857-56-4
  • MF: C22H18O6
  • MW: 378.37
  • Catalog: ROCK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Verosudil(AR-12286)

Verosudil (AR-12286) is a potent, selective Rho-kinase (ROCK) inhibitor with Kis of 2 and 2 nM for ROCK1 and ROCK2, respectively. AR-12286 lowers intraocular pressure (IOP) primarily by increasing aqueous humour outflow through the trabecular meshwork[1][2].

  • CAS Number: 1414854-42-4
  • MF: C17H17N3O2S
  • MW: 327.401
  • Catalog: ROCK
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 625.5±55.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 332.1±31.5 °C

BDP5290

BDP5290 is a potent inhibitor of both ROCK and MRCK with IC50s of 5 nM, 50 nM, 10 nM and 100 nM for ROCK1, ROCK2, MRCKα and MRCKβ, respectively.

  • CAS Number: 1817698-21-7
  • MF: C17H18ClN7O
  • MW: 371.82
  • Catalog: ROCK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

SAR407899 (hydrochloride)

SAR407899 hydrochloride is a selective, potent and ATP-competitive ROCK inhibitor, with an IC50 of 135 nM for ROCK-2, and Kis of 36 nM and 41 nM for human and rat ROCK-2, respectively.

  • CAS Number: 923262-96-8
  • MF: C14H17ClN2O2
  • MW: 280.75000
  • Catalog: ROCK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

ROCK-IN-9

ROCK-IN-9 (Compound T345) is a ROCK inhibitor. ROCK-IN-9 shows cytotoxicity in HepG2 cell, with an IC50 of 40.8 μM. ROCK-IN-9 has good pharmacokinetic properties in mice, and shows high in vivo exposure and oral bioavailability at lower doses[1].

  • CAS Number: 2643334-76-1
  • MF: C20H20FN5O2
  • MW: 381.40
  • Catalog: ROCK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

GSK269962 HCl

GSK269962A hydrochloride (GSK 269962 hydrochloride) is a potent ROCK inhibitor with IC50s of 1.6 and 4 nM for recombinant human ROCK1 and ROCK2 respectively. GSK269962A hydrochloride has anti-inflammatory and vasodilatory activities[1].

  • CAS Number: 2095432-71-4
  • MF: C29H31ClN8O5
  • MW: 607.06
  • Catalog: ROCK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

SR-3677

SR-3677 is a potent and selective ROCK-II inhibitor with an IC50 of ~3 nM.

  • CAS Number: 1072959-67-1
  • MF: C22H24N4O4
  • MW: 408.450
  • Catalog: Autophagy
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 646.9±55.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 345.0±31.5 °C

HA-100 hydrochloride

HA-100 dihydrochloride is a potent protein kinase inhibitor, with IC50s of 4 μM, 8 μM, 12 μM and 240 μM for cGMP-dependent protein kinase (PKG), cAMP-dependent protein kinase (PKA), protein kinase C (PKC) and MLC-kinase, respectively. HA-100 dihydrochloride also used as a ROCK inhibitor[1][2].

  • CAS Number: 210297-47-5
  • MF: C13H17Cl2N3O2S
  • MW: 350.26
  • Catalog: ROCK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Chroman 1

Chroman 1 is a highly potent ROCK2 inhibitor, with an IC50 of 1 nM.

  • CAS Number: 1273579-40-0
  • MF: C24H28N4O4
  • MW: 436.50400
  • Catalog: ROCK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Fasudil

Fasudil is a potent inhibitor of ROCK1, PKA, PKC, and MLCK with Ki of 0.33 μM, 1.0 μM, 9.3 μM and 55 μM, respectively.

  • CAS Number: 103745-39-7
  • MF: C14H17N3O2S
  • MW: 291.37
  • Catalog: Autophagy
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 506.2±60.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 259.9±32.9 °C

ROCK-IN-6

ROCK-IN-6 is a potent and selectiveROCK2 inhibitor with an IC50 of 2.19 nM. ROCK-IN-6 is extracted from patent WO2021164351 A1, example 7, has the potential for glaucoma and retinal diseases research[1].

  • CAS Number: 2489328-74-5
  • MF: C19H19N5O8S
  • MW: 477.45
  • Catalog: ROCK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

CCG-232964

CCG-232964 is an orally active inhibitor of Rho/MRTF/SRF. CCG-232964 inhibits LPA-induced CTGF gene expression[1].

  • CAS Number: 2349373-70-0
  • MF: C15H15ClN2O3S
  • MW: 338.81
  • Catalog: ROCK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Belumosudil mesylate

Belumosudil mesylate (KD025 mesylate) is a selective inhibitor of ROCK2 with IC50s of 105 nM and 24 µM for ROCK2 and ROCK1, respectively. Anti-fibrotic properties[1].

  • CAS Number: 2109704-99-4
  • MF: C27H28N6O5S
  • MW: 548.61
  • Catalog: ROCK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

1-amino-8-naphthol-2,4-disulfonic acid monosodium salt

CaMKP inhibitor sodium is a selective inhibitor of ROCK1 with an IC50 value of 14 nM.

  • CAS Number: 52789-62-5
  • MF: C10H8NNaO7S2
  • MW: 341.29
  • Catalog: ROCK
  • Density: 1.477 g/mL at 25 °C(lit.)
  • Boiling Point: 270-272 °C(lit.)
  • Melting Point: 12-16 °C(lit.)
  • Flash Point: >230 °F

ROCK-IN-8

ROCK-IN-8 (Example 4) is a ROCK inhibitor, with an IC50 value less than 100 nM. ROCK-IN-8 has anti-inflammatory activity. ROCK-IN-8 can be used for research of respiratory and gastro-intestinal diseases[1].

  • CAS Number: 1621103-79-4
  • MF: C30H25FN4O4S
  • MW: 556.61
  • Catalog: ROCK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

K-115 (free base)

Ripasudil free base (K-115 free base) is a specific inhibitor of ROCK, with IC50s of 19 and 51 nM for ROCK2 and ROCK1, respectively.

  • CAS Number: 223645-67-8
  • MF: C15H18FN3O2S
  • MW: 323.386
  • Catalog: ROCK
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 497.2±55.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 254.5±31.5 °C

AT13148

AT13148 is an orally active and ATP-competitive, multi-AGC kinase inhibitor with IC50s of 38 nM/402 nM/50 nM, 8 nM, 3 nM, and 6 nM/4 nM for Akt1/2/3, p70S6K, PKA, and ROCKI/II, respectively.

  • CAS Number: 1056901-62-2
  • MF: C17H16ClN3O
  • MW: 313.78
  • Catalog: ROCK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Y-33075

Y-33075 is a selective ROCK inhibitor derived from Y-27632, and is more potent than Y-27632, with an IC50 of 3.6 nM.

  • CAS Number: 199433-58-4
  • MF: C16H16N4O
  • MW: 280.32400
  • Catalog: ROCK
  • Density: 1.32
  • Boiling Point: 444.644ºC at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 222.713ºC

ROCK inhibitor-2

ROCK inhibitor-2 is a selective dual ROCK1 and ROCK2 inhibitor with IC50s of 17 nM and 2 nM, respectively[1].

  • CAS Number: 1127308-52-4
  • MF: C21H20N2O2
  • MW: 332.40
  • Catalog: ROCK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

RKI-1313

RKI-1313 is a ROCK inhibitor with IC50s of 34, 8 µM for ROCK 1 and ROCK 2, respectively. RKI-1313 shows little effect on the phosphorylation levels of ROCK substrates, migration, invasion or anchorage-independent growth[1].

  • CAS Number: 1342276-76-9
  • MF: C17H16N4O2S
  • MW: 340.400
  • Catalog: ROCK
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

4-(4-chloro-2-fluorophenyl)-2-(2-chloropyridin-4-yl)-1-(6-fluoro-1H-indazol-5-yl)-6-methyl-4H-pyrimidine-5-carboxamide

GSK-25 is a potent, selective and orally bioavailable ROCK1 inhibitor (IC50=7 nM). GSK-25 maintains good selectivity against a panel of 31 kinases (>100 fold), as well as RSK1 and p70S6K (RSK1: IC50=398 nM, p70S6K: IC50=1 μM). GSK-25 inhibits P450 profile (IC50s of 2.5, 5.2, 2.5 µM for CYP2C9, CYP2D6, CYP3A4, respectively)[1].

  • CAS Number: 874119-56-9
  • MF: C24H16Cl2F2N6O
  • MW: 513.326
  • Catalog: ROCK
  • Density: 1.6±0.1 g/cm3
  • Boiling Point: 751.7±60.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 408.4±32.9 °C

GSK180736A

GSK180736A is a G protein-coupled receptor kinase 2 (GRK2) inhibitor with an IC50 of 0.77 μM.

  • CAS Number: 817194-38-0
  • MF: C19H16FN5O2
  • MW: 365.3661
  • Catalog: ROCK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

ROCK2-IN-7

ROCK2-IN-7 is a kinase inhibitor targeting to ROCK2. ROCK2-IN-7 inhibits ROCK2/pSTAT3 Signaling. ROCK2-IN-7 suppresses systemic immunity activation and attenuates inflammation in psoriasis model[1].

  • CAS Number: 3000541-95-4
  • MF: C26H28FN5O
  • MW: 445.53
  • Catalog: ROCK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

GSK 269962

GSK269962A is a potent ROCK inhibitor with IC50s of 1.6 and 4 nM for recombinant human ROCK1 and ROCK2 respectively.

  • CAS Number: 850664-21-0
  • MF: C29H30N8O5
  • MW: 570.59900
  • Catalog: ROCK
  • Density: 1.45
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

ROCK2-IN-6

ROCK2-IN-6 (Comp A) is a selective ROCK2 inhibitor, can be used for ROCK mediated diseases, autoimmune diseases and inflammation research[1].

  • CAS Number: 2260506-59-8
  • MF: C26H21F2N7O
  • MW: 485.49
  • Catalog: ROCK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A