Cell Cycle includes many processes necessary for successful self-replication, and consists of DNA synthesis (S) and mitosis (M) phases separated by gap phases in the order G1–S–G2–M. S phase and M phase are usually separated by gap phases called G1 and G2, when cell-cycle progression can be regulated by various intracellular and extracellular signals. In order to move from one phase of its life cycle to the next, a cell must pass through numerous checkpoints. At each checkpoint, specialized proteins determine whether the necessary conditions exist. Progression through G1 phase is controlled by pRB proteins, and phosphorylation of pRB proteins by CDKs releases E2F factors, promoting the transition to S phase. The G2/M transition that commits cells to division is a default consequence of initiating the cell cycle at the G1/S transition, many proteins, such Wee1, PLK1 and cdc25, is involved the regulation of this process. The best-understood checkpoints are those activated by DNA damage and problems with DNA replication.

DNA damage response (DDR) is a series of regulatory events including DNA damage, cell-cycle arrest, regulation of DNA replication, and repair or bypass of DNA damage to ensure the maintenance of genomic stability and cell viability. Genome instability arises if cells initiate mitosis when chromosomes are only partially replicated or are damaged by a double-strand DNA break (DSB). To prevent cells with damaged DNA from entering mitosis, ATR inhibits cyclin B/Cdk1 activation by stimulating the Cdk1 inhibitory kinase Wee1 and inhibiting Cdc25C via Chk1, besides, ATM and ATR also initiate DNA repair by phosphorylating several other substrates.

In cancer cells, the cell cycle regulators as well as other elements of the DDR pathway have been found to protect tumor cells from different stresses and to promote tumor progression. Thus, cell cycle proteins that directly regulate cell cycle progression (such as CDKs), as well as checkpoint kinases, Aurora kinases and PLKs, are promising targets in cancer therapy.

References:
[1] Rhind N, et al. Cold Spring Harb Perspect Biol. 2012 Oct; 4(10): a005942.
[2] Duronio RJ, et al. Cold Spring Harb Perspect Biol. 2013 Mar; 5(3): a008904.
[3] Liu W, et al. Mol Cancer. 2017 Mar 14;16(1):60.
[4] Ghelli Luserna di Rora' A, et al. J Hematol Oncol. 2017 Mar 29;10(1):77.


Anti-infection >
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2’-Deoxy-2’-fluoro-5-methylcytidine

2’-Deoxy-2’-fluoro-5-methylcytidine is a cytidine nucleoside analog. Cytidine analogs have a mechanism of inhibiting DNA methyltransferases (such as Zebularine, HY-13420), and have potential anti-metabolic and anti-tumor activities[1].

  • CAS Number: 182495-80-3
  • MF: C10H14FN3O4
  • MW: 259.23
  • Catalog: Nucleoside Antimetabolite/Analog
  • Density: 1.7±0.1 g/cm3
  • Boiling Point: 495.4±55.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 253.4±31.5 °C

UNII:2V3E7D3089

Rosiglitazone (BRL 49653) potassium is an orally active selective PPARγ agonist (EC50: 60 nM, Kd: 40 nM). Rosiglitazone potassium is a TRPC5 activator (EC50: 30 μM) and TRPM3 inhibitor. Rosiglitazone potassium can be used in the research of obesity and diabetes, senescence, ovarian cancer[1][2][4][7].

  • CAS Number: 316371-84-3
  • MF: C18H18KN3O3S
  • MW: 395.517
  • Catalog: Apoptosis
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

PNU112455A hydrochloride

PNU112455A hydrochloride is an ATP-competitive CDK2 and CDK5 inhibitor. PNU112455A hydrochloride binds to the ATP site of CDK2 and CDK5 with Kms of 3.6 and 3.2 μM, respectively [1].

  • CAS Number: 21886-12-4
  • MF: C10H12ClN5O2S
  • MW: 301.75300
  • Catalog: CDK
  • Density: 1.532g/cm3
  • Boiling Point: 568.6ºC at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 297.7ºC

2-Amino-N,N-dimethyl-2′-C-methyladenosine

2-Amino-N,N-dimethyl-2′-C-methyladenosine is a purine nucleoside analogue. Purine nucleoside analogs have broad antitumor activity targeting indolent lymphoid malignancies. Anticancer mechanisms in this process rely on inhibition of DNA synthesis, induction of apoptosis, etc[1].

  • CAS Number: 1336975-57-5
  • MF: C13H20N6O4
  • MW: 324.34
  • Catalog: Nucleoside Antimetabolite/Analog
  • Density: 1.7±0.1 g/cm3
  • Boiling Point: 684.3±65.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 367.6±34.3 °C

CAM833

CAM833 (CAM-833) is a potent, specific chemical inhibitor of the RAD51-BRCA2 interaction and RAD51 oligomerization with Kd of 366 nM;CAM833 inhibited RAD51 foci formation 6 h after exposure to 3 Gy IR, in a concentration-dependent manner with an IC50 of 6 uM, causeed a concentration-dependent decrease in RAD51 foci accompanied by increased DNA damage in A549 cells.CAM833 inhibted RAD51 molecular clustering at DNA damage sites visualized by SMLM, suppressed homologous recombination and potentiated cell-cycle arrest.CAM833 potentiated the growth suppressive effect of PARP1 inhibition in BRCA2 wild-type cells, as well as dose-dependent growth inhibition when combined with ionizing radiation.

  • CAS Number: 2758364-02-0
  • MF: C27H29FN4O5
  • MW: 508.55
  • Catalog: Apoptosis
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

6-Mercaptopurine monohydrate

6-Mercaptopurine hydrate is a purine analogue which acts as an antagonist of the endogenous purines and has been widely used as antileukemic agent and immunosuppressive drug.

  • CAS Number: 6112-76-1
  • MF: C5H6N4OS
  • MW: 170.192
  • Catalog: Nucleoside Antimetabolite/Analog
  • Density: N/A
  • Boiling Point: 490.6ºC at 760 mmHg
  • Melting Point: >300 °C(lit.)
  • Flash Point: 250.5ºC

Diethyl 1-benzyl-4-phenyl-1,4-dihydropyridine-3,5-dicarboxylate

BML-278 is a SIRT1 activator (EC150: 1 μM). BML-278 increases H3K9 methylation and inhibits H3K9 acetylation in both the paternal and maternal pronucleus. BML-278 improves early embryonic development. BML-278 arrests the cell cycle at the G1/S phase, and reduces senescence in primary human mesenchymal cells. BML-278 reduces tubulin acetylation in U937 cells. BML-278 also increases mitochondrial density in murine C2C12 myoblasts[1][2].

  • CAS Number: 120533-76-8
  • MF: C24H25NO4
  • MW: 391.46
  • Catalog: Sirtuin
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Irinotecan hydrochloride

Irinotecan hydrochloride is a water soluble topoisomerase I inhibitor mainly used to treat colon cancer and rectal cancer.

  • CAS Number: 100286-90-6
  • MF: C33H39ClN4O6
  • MW: 623.139
  • Catalog: Autophagy
  • Density: N/A
  • Boiling Point: 257 °C
  • Melting Point: 250-256°C (dec.)
  • Flash Point: 482ºC

Fmoc-leucine-d10

Fmoc-leucine-d10 is the deuterium labeled Fmoc-leucine. Fmoc-leucine is a selective PPARγ modulator. Fmoc-leucine activates PPARγ with a lower potency but a similar maximal efficacy than rosiglitazone. Fmoc-leucine improves insulin sensitivity in normal, diet-induced glucose-intolerant, and in diabetic db/db mice. Fmoc-leucine has a lower adipogenic activity[1].

  • CAS Number: 1190594-22-9
  • MF: C21H13D10NO4
  • MW: 363.47
  • Catalog: PPAR
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 559.8±33.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 292.4±25.4 °C

Cdc7-IN-12

Cdc7-IN-12 (compound 1) is a potent CDC7 inhibitor with an IC50 of <1 nM. Cdc7-IN-12 shows antiproliferative activities with IC50 of 100-1000 nM in COLO205 cells. Cdc7-IN-12 has the potential for the research of cancer[1].

  • CAS Number: 2764865-33-8
  • MF: C16H14N2O2S
  • MW: 298.36
  • Catalog: CDK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

2’,3’,5’-Tri-O-acetyl-5,N3-dimethyluridine

2’,3’,5’-Tri-O-acetyl-5,N3-dimethyluridine is a thymidine analog. Analogs of this series have insertional activity towards replicated DNA. They can be used to label cells and track DNA synthesis[1].

  • CAS Number: 201421-00-3
  • MF: C17H22N2O9
  • MW: 398.36
  • Catalog: Nucleoside Antimetabolite/Analog
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

TH34

TH34, an HDAC6/8/10 inhibitor with IC50s of 4.6 μM, 1.9 μM, and 7.7 μM respectively, shows high selectivity over HDAC1/2/3[1].

  • CAS Number: 2196203-96-8
  • MF: C15H16N2O2
  • MW: 256.30
  • Catalog: HDAC
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Pomiferin

Pomiferin, a flavonoid from the fruits of Maclura pomifera, acts as an potential inhibitor of HDAC, with an IC50 of 1.05 μM, and also potently inhibits mTOR (IC50, 6.2 µM).

  • CAS Number: 572-03-2
  • MF: C25H24O6
  • MW: 420.45400
  • Catalog: HDAC
  • Density: 1.314g/cm3
  • Boiling Point: 673.5ºC at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 233.3ºC

2',3',5'-Tri-O-benzoyl-2'-beta-C-methyl-6-azauridine

2',3',5'-Tri-O-benzoyl-2'-beta-C-methyl-6-azauridine is a purine nucleoside analog. Purine nucleoside analogs have broad antitumor activity targeting indolent lymphoid malignancies. Anticancer mechanisms in this process rely on inhibition of DNA synthesis, induction of apoptosis, etc[1].

  • CAS Number: 622379-83-3
  • MF: C31H27N3O9
  • MW: 585.56
  • Catalog: Nucleoside Antimetabolite/Analog
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

HDAC6-IN-26

HDAC6-IN-26 (compound 23) is a potent inhibitor of HDAC6[1].

  • CAS Number: 2991427-19-9
  • MF: C13H12N8O
  • MW: 296.29
  • Catalog: HDAC
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

SRPIN340

SRPIN340 is an ATP-competitive serine-arginine-rich protein kinase (SRPK) inhibitor, with a Ki of 0.89 μM for SRPK1.

  • CAS Number: 218156-96-8
  • MF: C18H18F3N3O
  • MW: 349.350
  • Catalog: SRPK
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 395.9±42.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 193.3±27.9 °C

1,N6-Etheno-9-(β-D-xylofuranosyl)adenosine

1,N6-Etheno-9-(β-D-xylofuranosyl)adenosine is a purine nucleoside analogue. Purine nucleoside analogs have broad antitumor activity targeting indolent lymphoid malignancies. Anticancer mechanisms in this process rely on inhibition of DNA synthesis, induction of apoptosis, etc[1].

  • CAS Number: 2095417-39-1
  • MF: C12H13N5O4
  • MW: 291.26
  • Catalog: Nucleoside Antimetabolite/Analog
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

1,3,5-Tri-O-benzoyl-α-D-ribofuranose

1,3,5-Tri-O-benzoyl-a-D-ribofuranose is a purine nucleoside analog. Purine nucleoside analogs have broad antitumor activity targeting indolent lymphoid malignancies. Anticancer mechanisms in this process rely on inhibition of DNA synthesis, induction of apoptosis, etc[1].

  • CAS Number: 22224-41-5
  • MF: C26H22O8
  • MW: 462.45
  • Catalog: Nucleoside Antimetabolite/Analog
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: 629.6±55.0 °C at 760 mmHg
  • Melting Point: 125-129 °C(lit.)
  • Flash Point: 213.0±25.0 °C

2,8-Dimethyladenosine

2,8-Dimethyladenosine is an adenosine analogue. Adenosine analogs mostly act as smooth muscle vasodilators and have also been shown to inhibit cancer progression. The popular products in this series are adenosine phosphate, Acadesine (HY-13417), Clofarabine (HY-A0005), Fludarabine phosphate (HY-B0028) and Vidarabine (HY-B0277)[1].

  • CAS Number: 63954-66-5
  • MF: C12H17N5O4
  • MW: 295.29
  • Catalog: Nucleoside Antimetabolite/Analog
  • Density: 1.9±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

1,2-Di-O-acetyl-5-Benzoyl-3-O-Methyl-D-ribofuranose

1,2-Di-O-acetyl-5-Benzoyl-3-O-Methyl-D-ribofuranose diacetate is a purine nucleoside analog. Purine nucleoside analogs have broad antitumor activity targeting indolent lymphoid malignancies. Anticancer mechanisms in this process rely on inhibition of DNA synthesis, induction of apoptosis, etc[1].

  • CAS Number: 10300-21-7
  • MF: C17H20O8
  • MW: 352.34
  • Catalog: Nucleoside Antimetabolite/Analog
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

GCN2iB

GCN2iB is an ATP-competitive inhibitor of a serine/threonine-protein kinase general control nonderepressible 2 (GCN2), with an IC50 of 2.4 nM.

  • CAS Number: 2183470-12-2
  • MF: C18H12ClF2N5O3S
  • MW: 451.83
  • Catalog: Eukaryotic Initiation Factor (eIF)
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Rucaparib camsylate

Rucaparib is an inhibitor of PARP with a Ki of 1.4 nM for PARP1, and also shows binding affinity to eight other PARP domains.

  • CAS Number: 1859053-21-6
  • MF: C29H34FN3O5S
  • MW: 555.661
  • Catalog: PARP
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

3-Phenyltoxoflavin

3-Phenyltoxoflavin, a derivative of Toxoflavin, is an Hsp90 inhibitor, with a Kd of 585 nM for the interaction of Hsp90-TPR2A. 3-Phenyltoxoflavin has anti-cancer activity[1][2].

  • CAS Number: 32502-63-9
  • MF: C13H11N5O2
  • MW: 269.25900
  • Catalog: HSP
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

CDK1/Cyc B-IN-1

CDK1/Cyc B-IN-1 (Compound 5) is a selective CDK1/Cyc B complex inhibitor with an IC50 of 97 nM. CDK1/Cyc B-IN-1 triggers apoptosis and G2/M cell cycle arrest. CDK1/Cyc B-IN-1 shows broad-spectrum cytotoxic action against cancer cell lines[1].

  • CAS Number: 2459916-56-2
  • MF: C14H12ClN3O2S2
  • MW: 353.85
  • Catalog: Apoptosis
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

2'-Fluoro-5'-O-DMT-2'-deoxyinosine-3'-CE-phosphoramidite

2'-Fluoro-5'-O-DMT-2'-deoxyinosine-3'-CE-phosphoramidite is a hypoxanthine analog. Hypoxanthine is a kind of purine base mainly present in muscle tissue. And it is a metabolite produced by purine oxidase acting on xanthine. Hypoxanthine has typical anti-inflammatory effects and is a potential endogenous poly(ADP-ribose) polymerase (PARP) inhibitor. It is cytoprotective by inhibiting PAPR activity, inhibiting peroxynitrite-induced mitochondrial depolarization and secondary superoxide production. Hypoxanthine can also be used as an indicator of hypoxia[1][2].

  • CAS Number: 2245842-16-2
  • MF: C40H46FN6O7P
  • MW: 772.80
  • Catalog: Nucleoside Antimetabolite/Analog
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

H-1152

H-1152 is a membrane-permeable and selective ROCK inhibitor, with a Ki value of 1.6 nM, and an IC50 value of 12 nM for ROCK2.

  • CAS Number: 451462-58-1
  • MF: C16H21N3O2S
  • MW: 319.42
  • Catalog: ROCK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

KIF18A-IN-2

KIF18A-IN-2 is a potent KIF18A inhibitor (IC50=28 nM). KIF18A-IN-2 causes significant mitotic arrest and increases the number of mitotic cells in tumor tissues. KIF18A-IN-2 can be used for researching cancer[1].

  • CAS Number: 2600559-20-2
  • MF: C25H34N4O5S2
  • MW: 534.69
  • Catalog: Microtubule/Tubulin
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

2’-O-Acetyl-5’-O-(p-toluoyl)-3’-deoxy-3’-fluoro-5-methyluridine

2’-O-Acetyl-5’-O-(p-toluoyl)-3’-deoxy-3’-fluoro-5-methyluridine is a purine nucleoside analog. Purine nucleoside analogs have broad antitumor activity targeting indolent lymphoid malignancies. Anticancer mechanisms in this process rely on inhibition of DNA synthesis, induction of apoptosis, etc[1].

  • CAS Number: 2072145-23-2
  • MF: C20H21FN2O7
  • MW: 420.39
  • Catalog: Nucleoside Antimetabolite/Analog
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

N6-Dimethylamino methylidene-2’-deoxyisoguanosine

N6-Dimethylamino methylidene-2’-deoxyisoguanosine is a purine nucleoside analog. Purine nucleoside analogs have broad antitumor activity targeting indolent lymphoid malignancies. Anticancer mechanisms in this process rely on inhibition of DNA synthesis, induction of apoptosis, etc[1].

  • CAS Number: 146196-17-0
  • MF: C13H18N6O4
  • MW: 322.32
  • Catalog: Nucleoside Antimetabolite/Analog
  • Density: 1.7±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Metribuzin

Metribuzin is a low-cost non-selective herbicide that belongs to the chemical class of triazinones. Metribuzin hinders DNA synthesis in treated plants and acts on photosystem II, ultimately inhibiting photosynthesis. Metribuzin provides good control of important annual grass and broad-leaf weeds[1].

  • CAS Number: 21087-64-9
  • MF: C8H14N4OS
  • MW: 214.288
  • Catalog: DNA/RNA Synthesis
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 312.4±25.0 °C at 760 mmHg
  • Melting Point: 125°C
  • Flash Point: 142.7±23.2 °C