A dye is a colored substance that has an affinity to the substrate to which it is being applied.

A new approach is presented for analysis of microplastics in environmental samples, based on selective fluorescent staining using Nile Red (NR), followed by density-based extraction and filtration. The dye adsorbs onto plastic surfaces and renders them fluorescent when irradiated with blue light. Fluorescence emission is detected using simple photography through an orange filter. Image-analysis allows fluorescent particles to be identified and counted. Magnified images can be recorded and tiled to cover the whole filter area, allowing particles down to a few micrometres to be detected. The solvatochromic nature of Nile Red also offers the possibility of plastic categorisation based on surface polarity characteristics of identified particles. This article details the development of this staining method and its initial cross-validation by comparison with infrared (IR) microscopy. Microplastics of different sizes could be detected and counted in marine sediment samples. The fluorescence staining identified the same particles as those found by scanning a filter area with IR-microscopy.

Cyanine dyes can be used to quantify the amount of dsDNA within a sample. The linearity of fluorescence, as function of DNA amount of six dyes, is obtained by measuring the fluorescence intensity at the optimal excitation and emission maxima.


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Tartrazine

Tartrazine is a synthetic lemon yellow azo dye primarily used as a food coloring. Tartrazine is water-soluble and has a maximum absorbance in an aqueous solution at 425 nm.

  • CAS Number: 1934-21-0
  • MF: C16H9N4Na3O9S2
  • MW: 534.36
  • Catalog: Dye Reagents
  • Density: 1.93 g/cm3 (20ºC)
  • Boiling Point: N/A
  • Melting Point: 300ºC
  • Flash Point: N/A

Fluo-3AM

Fluo-3AM(Fluo-3-pentaacetoxymethyl ester) is a calcium indicator that exhibit an increase in fluorescence upon binding Ca2+; used to image the spatial dynamics of Ca2+ signaling, in flow cytometry experiments involving photoactivation of caged chelators, second messengers, and neurotransmitters, and for cell-based pharmacological screening.

  • CAS Number: 121714-22-5
  • MF: C51H50Cl2N2O23
  • MW: 1129.85
  • Catalog: Dye Reagents
  • Density: 1.5±0.1 g/cm3
  • Boiling Point: 1075.8±65.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 604.4±34.3 °C

7-Amino-4-methylcoumarin

7-amino-4-methylcoumarin has been used as a fluorogenic probe for many serine proteases.

  • CAS Number: 26093-31-2
  • MF: C10H9NO2
  • MW: 175.184
  • Catalog: Dye Reagents
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 378.3±37.0 °C at 760 mmHg
  • Melting Point: 223-226 °C(lit.)
  • Flash Point: 216.9±24.0 °C

Solvent Green 7

Pyranine as a new class of fluorescent chemosensor for the Cu+ ion is reported.

  • CAS Number: 6358-69-6
  • MF: C16H7Na3O10S3
  • MW: 524.385
  • Catalog: Dye Reagents
  • Density: 2.15
  • Boiling Point: 171-178 °C10 mm Hg(lit.)
  • Melting Point: 62-63.5 °C(lit.)
  • Flash Point: 250 °C

CY7

Cy7 dyes are widely used for labeling peptides, proteins and oligos etc. Cy7 dye conjugates are one type of the most common near infrared red fluorophores used in in vivo imaging applications. Excitation (nm): 749, Emission (nm): 767

  • CAS Number: 943298-08-6
  • MF: C35H42N2O8S2
  • MW: 682.84700
  • Catalog: Dye Reagents
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Coelenterazine

Coelenterazine is widely distributed among marine organisms which can produce bioluminescence by calcium-dependent oxidation mediated by the photoprotein aequorin.

  • CAS Number: 55779-48-1
  • MF: C26H21N3O3
  • MW: 423.463
  • Catalog: Dye Reagents
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 641.4±65.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 341.7±34.3 °C

2-NBDG

2-NBDG is a fluorescent indicator for direct glucose uptake measurement and also is an indicator of cell viability.

  • CAS Number: 186689-07-6
  • MF: C12H14N4O8
  • MW: 342.262
  • Catalog: Dye Reagents
  • Density: 1.750±0.06 g/cm3
  • Boiling Point: 707.6±70.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 381.7±35.7 °C

Acridine Orange hydrochloride

Acridine orange is a cell-permeable fluorescent dye that binds to nucleic acids, resulting in an altered spectral emission.

  • CAS Number: 65-61-2
  • MF: C17H20ClN3
  • MW: 301.81400
  • Catalog: Dye Reagents
  • Density: 1.001 g/mL at 20ºC
  • Boiling Point: 468.6ºC at 760 mmHg
  • Melting Point: 284-287ºC(lit.)
  • Flash Point: 237.2ºC

Hydroxystilbamidine bis(methanesulfonate)

Hydroxystilbamidine bis(methanesulfonate), a dye capable of binding to both DNA and RNA, has been found to be a powerful inhibitor of cellular ribonucleases.

  • CAS Number: 223769-64-0
  • MF: C18H24N4O7S2
  • MW: 472.536
  • Catalog: Dye Reagents
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

D-Luciferin

D-luciferin is the natural substrate of luciferases that catalyze the production of light in bioluminescent insects.

  • CAS Number: 2591-17-5
  • MF: C11H8N2O3S2
  • MW: 280.323
  • Catalog: Dye Reagents
  • Density: 1.8±0.1 g/cm3
  • Boiling Point: 587.6±60.0 °C at 760 mmHg
  • Melting Point: 200-204ºC
  • Flash Point: 309.2±32.9 °C

Fluorescamine

Fluorescamine( Ro 20-7234) is a spiro compound that is not fluorescent itself, but reacts with primary amines to form highly fluorescent products. It hence has been used as a reagent for the detection of amines and peptides.

  • CAS Number: 38183-12-9
  • MF: C17H10O4
  • MW: 278.259
  • Catalog: Dye Reagents
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: 550.3±50.0 °C at 760 mmHg
  • Melting Point: 153-157 °C(lit.)
  • Flash Point: 248.5±30.2 °C

TMB-PS

TMB-PS(102062-36-2) is N-(3-sulfopropyl)-3,3',5,5'-tetramenthylbenzidine sodium salt; White - pale yellow crystalline powder, soluble in water, can be used under neutral, acidic and alkaline conditions.

  • CAS Number: 102062-36-2
  • MF: C19H26N2O3S
  • MW: 362.486
  • Catalog: Dye Reagents
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: 230-240ºC
  • Flash Point: N/A

5-ROX

5-ROX is a pure-5 isomer derived from carboxy-X-rhodamine. 5-ROX is a fluorescent oligonucleotide marker, acts as a donor molecule in FRET imaging coupled with porphyrins.5-ROX is widely used for oligonucleotide labeling and automated DNA sequencing applications.Excitation:575nm. Emission:575nm

  • CAS Number: 216699-35-3
  • MF: C33H30N2O5
  • MW: 534.60200
  • Catalog: Dye Reagents
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

LUMINOL SODIUM SALT

Luminol sodium salt is a versatile chemical that exhibits chemiluminescence, with a striking blue glow, when mixed with an appropriate oxidizing agent.

  • CAS Number: 20666-12-0
  • MF: C8H7N3NaO2+
  • MW: 200.15
  • Catalog: Dye Reagents
  • Density: 1.433g/cm3
  • Boiling Point: 621.9ºC at 760mmHg
  • Melting Point: 319-320ºC
  • Flash Point: 329.9ºC

TRx0237 (LMTX) mesylate

Leucomethylene blue (Mesylate) is a common reduced form of Methylene Blue, Methylene Blue is a member of the thiazine class of dyes. Target:Methylene blue has long been used as an antiseptic and therapeutic agent with both human and veterinary applications.

  • CAS Number: 1236208-20-0
  • MF: C18H27N3O6S3
  • MW: 477.618
  • Catalog: Dye Reagents
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Phenol Red sodium salt

Phenol Red sodium salt is a water soluble pH indicator used in the 6.8 (yellow) to 8.2 (red) range.

  • CAS Number: 34487-61-1
  • MF: C19H13NaO5S
  • MW: 375.351
  • Catalog: Dye Reagents
  • Density: 0.972
  • Boiling Point: 562.8ºC at 760mmHg
  • Melting Point: 285 °C (dec.)(lit.)
  • Flash Point: 294.2ºC

Hoechst 33258 analog 6

Hoechst 33258 analog 6 is a anglog of Hoechst stains(Hoechst 33258), which are part of a family of blue fluorescent dyes used to stain DNA.IC50 Value:Target: These Bis-benzimides were originally developed by Hoechst AG, which numbered all their compounds so that the dye Hoechst 33342 is the 33342nd compound made by the company. There are three related Hoechst stains: Hoechst 33258, Hoechst 33342, and Hoechst 34580. The dyes Hoechst 33258 and Hoechst 33342 are the ones most commonly used and they have similarexcitation/emission spectra. Both dyes are excited by ultraviolet light at around 350 nm, and both emit blue/cyan fluorescent light around anemission maximum at 461 nm. Unbound dye has its maximum fluorescence emission in the 510-540 nm range. Hoechst dyes are soluble in water and in organic solvents such as dimethyl formamide or dimethyl sulfoxide. Concentrations can be achieved of up to 10 mg/mL. Aqueous solutions are stable at 2-6 °C for at least six months when protected from light. For long-term storage the solutions are instead frozen at ≤-20 °C.The dyes bind to the minor groove of double-stranded DNA with a preference for sequences rich in adenine andthymine. Although the dyes can bind to all nucleic acids, AT-rich double-stranded DNA strands enhance fluorescence considerably.Hoechst dyes are cell-permeable and can bind to DNA in live or fixed cells. Therefore, these stains are often called supravital, which means that cells survive a treatment with these compounds. Cells that express specific ATP-binding cassette transporter proteins can also actively transport these stains out of their cytoplasm.

  • CAS Number: 129244-66-2
  • MF: C33H40N6O
  • MW: 536.71000
  • Catalog: Dye Reagents
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

FHZ

FHZ is a fluorescent probe.

  • CAS Number: 1883737-63-0
  • MF: C26H26N2O7
  • MW: 478.49
  • Catalog: Dye Reagents
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

6-TAMRA-SE

6-TAMRA-SE is an amine-reactive form of tetramethylrhodamine; one of the traditional fluorophores used for automated DNA sequencing.

  • CAS Number: 150810-69-8
  • MF: C29H25N3O7
  • MW: 527.525
  • Catalog: Dye Reagents
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

5(6)-Carboxyfluorescein

5(6)-Carboxyfluorescein contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid; cell-impermeant 5,6-FAM can also be used as a nonfixable polar tracer to investigate fusion, lysis and gap-junctional communication and to detect changes in cell or liposome volume.

  • CAS Number: 72088-94-9
  • MF: C21H12O7
  • MW: 376.32
  • Catalog: Dye Reagents
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: 275 °C
  • Flash Point: N/A

Luminol

Luminol(Diogenes reagent) is a versatile chemical that exhibits chemiluminescence, with a striking blue glow, when mixed with an appropriate oxidizing agent.

  • CAS Number: 521-31-3
  • MF: C8H7N3O2
  • MW: 177.160
  • Catalog: Dye Reagents
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: 621.9ºC at 760 mmHg
  • Melting Point: >300 °C(lit.)
  • Flash Point: 329.9ºC

DMA

DMA is a fluorescent compound (λex=340 nm, λem=478 nm).

  • CAS Number: 188860-26-6
  • MF: C27H28N6O2
  • MW: 468.55000
  • Catalog: Dye Reagents
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

1-Methyl-7-nitroisatoic anhydride

1-methyl-7-nitroisatoic anhydride is a reagent that detects local nucleotide flexibility, for probing 2'-hydroxyl reactivity.

  • CAS Number: 73043-80-8
  • MF: C9H6N2O5
  • MW: 222.154
  • Catalog: Dye Reagents
  • Density: 1.6±0.1 g/cm3
  • Boiling Point: 395.4±44.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 192.9±28.4 °C

(±)-ANAP

(±)-ANAP is the unnatural amino acid analog of prodan, acts as a fluorescent probes, and enhances environmental sensitivity.

  • CAS Number: 1185251-08-4
  • MF: C15H16N2O3
  • MW: 272.29900
  • Catalog: Dye Reagents
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Indocyanine Green

Cardiogreen is a low toxicic fluorescent agent that has been widely used in medical diagnostics, such as determining cardiac output, hepatic function, and liver blood flow, and for ophthalmic angiography.

  • CAS Number: 3599-32-4
  • MF: C43H47N2NaO6S2
  • MW: 774.963
  • Catalog: Dye Reagents
  • Density: N/A
  • Boiling Point: 157ºC
  • Melting Point: 235 °C
  • Flash Point: N/A

5-carboxyfluorescein

The single isomer, 5-FAM, contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. Fluorescein is the most common fluorescent derivatization reagent for labeling biomolecules. In addition to its relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility, fluorescein has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser.

  • CAS Number: 76823-03-5
  • MF: C21H12O7
  • MW: 376.316
  • Catalog: Dye Reagents
  • Density: 1.7±0.1 g/cm3
  • Boiling Point: 725.2±60.0 °C at 760 mmHg
  • Melting Point: 368-372°C
  • Flash Point: 266.9±26.4 °C

ADOS

ADOS(82692-96-4) is a biochemical reagent/chromogenic reagent.Molar absorptivity(pH10): ≥8,200(around 255 nm)Appearance: White or slightly brown powder

  • CAS Number: 82692-96-4
  • MF: C12H18NNaO5S
  • MW: 311.33
  • Catalog: Dye Reagents
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: 144 °C
  • Flash Point: N/A

NucPE1

NucPE1 (Nuclear Peroxy Emerald 1) is a nuclear-localized fluorescent hydrogen peroxide that is specifically localized to cellular nuclei without appended targeting moieties.

  • CAS Number: 1404091-23-1
  • MF: C29H30BNO5
  • MW: 483.36
  • Catalog: Dye Reagents
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

ALPS

ALPS(N-Ethyl-N-sulfopropylaniline sodium salt) is a bio-chemical reagents/chromogenic reagent.Appearance: White or slightly grayish-yellow powderMolar absorptivity: ≥9,500 (around 255 nm)Application: Hydrogen peroxide detection, colorimetric

  • CAS Number: 82611-85-6
  • MF: C11H16NNaO3S
  • MW: 265.304
  • Catalog: Dye Reagents
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: 265 °C (dec.)(lit.)
  • Flash Point: N/A

Cresyl Violet perchlorate

Cresyl Violet perchlorate is a red fluorescent stain, which can be used to stain neurons.

  • CAS Number: 41830-80-2
  • MF: C16H12ClN3O5
  • MW: 361.73700
  • Catalog: Dye Reagents
  • Density: N/A
  • Boiling Point: 482.3ºC at 760mmHg
  • Melting Point: 331 - 333ºC
  • Flash Point: 245.5ºC