Metabolic pathways are enzyme-mediated biochemical reactions that lead to biosynthesis (anabolism) or breakdown (catabolism) of natural product small molecules within a cell or tissue. In each pathway, enzymes catalyze the conversion of substrates into structurally similar products. Metabolic processes typically transform small molecules, but also include macromolecular processes such as DNA repair and replication, and protein synthesis and degradation. Metabolism maintains the living state of the cells and the organism.

Proteases are used throughout an organism for various metabolic processes. Proteases control a great variety of physiological processes that are critical for life, including the immune response, cell cycle, cell death, wound healing, food digestion, and protein and organelle recycling. On the basis of the type of the key amino acid in the active site of the protease and the mechanism of peptide bond cleavage, proteases can be classified into six groups: cysteine, serine, threonine, glutamic acid, aspartate proteases, as well as matrix metalloproteases. Proteases can not only activate proteins such as cytokines, or inactivate them such as numerous repair proteins during apoptosis, but also expose cryptic sites, such as occurs with β-secretase during amyloid precursor protein processing, shed various transmembrane proteins such as occurs with metalloproteases and cysteine proteases, or convert receptor agonists into antagonists and vice versa such as chemokine conversions carried out by metalloproteases, dipeptidyl peptidase IV and some cathepsins. In addition to the catalytic domains, a great number of proteases contain numerous additional domains or modules that substantially increase the complexity of their functions.

Imbalances in metabolic activities have been found to be critical in a number of pathologies, such as cardiovascular diseases, inflammation, cancer, and neurodegenerative diseases.

References:
[1] Turk B, et al. EMBO J. 2012 Apr 4;31(7):1630-43.
[2] Eatemadi A, et al. Biomed Pharmacother. 2017 Feb;86:221-231.


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R 80123

R 80123 is the Z-isomer of R 79595, is also a highly selective phosphodiesterase inhibitor. The fuction is similar to R 80122 (HY-100615, Revizinone).In vivo: The administration of Revizinone improved the haemodynamic profile with an increase in cardiac output, a decrease in systemic vascular resistance and a stable heart rate and mean arterial blood pressure. [1] With regard to reconstitution of contractility and cardiac function Revizinone (E-isomer) is 10 fold more potent than R 79595 and nearly 100 fold more potent than R 80123 (Z-isomer). [2] Revizinone significantly increases global LV function and systolic wall thickening in ischemic areas at doses greater than or equal to 0.16 mg/kg i.v. [3]

  • CAS Number: 133718-30-6
  • MF: C26H29N5O3
  • MW: 459.540
  • Catalog: Phosphodiesterase (PDE)
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

YM17E

YM17E is an inhibitor of acyl CoA:cholesterol acyltransferase (ACAT), with IC50 of 44 nM in rabbit liver microsomes in vitro.

  • CAS Number: 124900-72-7
  • MF: C40H56N6O2
  • MW: 652.912
  • Catalog: Acyltransferase
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 862.7±65.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 475.5±34.3 °C

Skp2 inhibitor 1

Skp2 inhibitor 1 (compound 14i) is a potent and selective Skp2 inhibitor against the Skp2-Cks1 interaction with an IC50 of 2.8 μM. Skp inhibitor 1 exhibits anticancer activity[1].

  • CAS Number: 2760612-63-1
  • MF: C23H23ClN4O
  • MW: 406.91
  • Catalog: E1/E2/E3 Enzyme
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Berotralstat dihydrochloride

Berotralstat dihydrochloride is a low toxicity, effective, highly specific, second-generation, synthetic and orally active plasma kallikrein inhibitor used for the research of hereditary angioedema (HAE) attacks. Berotralstat dihydrochloride works by blocking the enzymatic activity of plasma kallikrein in releasing bradykinin, the major biologic peptide that promotes swelling and pain associated with attacks of HAE[1][2].

  • CAS Number: 1809010-52-3
  • MF: C30H28Cl2F4N6O
  • MW: 635.48
  • Catalog: Ser/Thr Protease
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Lys-Ala-pNA

Lys-Ala-pNA is hydrolytic substrate the of dipeptidyl peptidase (DPP) II with Km of 0.42 mM[1].

  • CAS Number: 115846-24-7
  • MF: C15H23N5O4
  • MW: 337.37
  • Catalog: Dipeptidyl Peptidase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

17alpha-Estradiol

Alpha-Estradiol is a weak estrogen and a 5α-reductase inhibitor which is used as a topical medication in the treatment of androgenic alopecia.

  • CAS Number: 57-91-0
  • MF: C18H24O2
  • MW: 272.382
  • Catalog: 5 alpha Reductase
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 445.9±45.0 °C at 760 mmHg
  • Melting Point: 176-180ºC(lit.)
  • Flash Point: 209.6±23.3 °C

Homo Sildenafil

Homo Sildenafil, an analog of Sildenafil, acts as a phosphodiesterase inhibitor[1].

  • CAS Number: 642928-07-2
  • MF: C23H32N6O4S
  • MW: 488.603
  • Catalog: Phosphodiesterase (PDE)
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: 200-202ºC
  • Flash Point: N/A

Quinaprilat

Quinaprilat hydrate is a non-mercapto Angiotensin Converting Enzyme (ACE) inhibitor, the active metabolite of Quinapril. Quinaprilat hydrate specifically blocks the conversion of angiotensin I to the vasoconstrictor angiotensin II and inhibits the degradation of bradykinin. Quinaprilat hydrate acts as anti-hypertensive agent and vasodilator[1][2].

  • CAS Number: 1435786-09-6
  • MF: C25H32N2O6
  • MW: 456.53100
  • Catalog: Angiotensin-converting Enzyme (ACE)
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Benzimidamide hydrochloride

Benzamidine hydrochloride is an reversible competitive inhibitor of trypsin-like serine proteases, with Kis of 97 µM, 21 µM, 20 µM and 110 µM for uPA, trypsin, tryptase and factor Xa, respectively[1].

  • CAS Number: 1670-14-0
  • MF: C7H9ClN2
  • MW: 156.613
  • Catalog: Ser/Thr Protease
  • Density: 1.09 g/cm3
  • Boiling Point: 332.9ºC at 760 mmHg
  • Melting Point: 86-88 °C(lit.)
  • Flash Point: 155.1ºC

Alamandine

Alamandine, a member of the renin-angiotensin system (RAS), a vasoactive peptide, is an endogenous ligand of the G protein-coupled receptor MrgD. Alamandine targets to protect the kidney and heart through anti-hypertensive actions[1][2].

  • CAS Number: 1176306-10-7
  • MF: C40H62N12O9
  • MW: 854.995
  • Catalog: Angiotensin Receptor
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Nitrefazole

Nitrefazole is a 4-nitroimidazole derivative with strong and long lasting inhibition of aldehyde dehydrogenase (ALDH), an enzyme involved in the metabolism of alcohol.

  • CAS Number: 21721-92-6
  • MF: C10H8N4O4
  • MW: 248.19500
  • Catalog: Aldehyde Dehydrogenase (ALDH)
  • Density: 1.53g/cm3
  • Boiling Point: 484.9ºC at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 247.1ºC

MMP-9-IN-3

MMP-9-IN-3 is a MMP-9 inhibitor (IC50: 5.56 nM) that forms hydrogen bond with MMP-9. MMP-9-IN-3 also inhibits AKT activity (IC50: 2.11 nM). MMP-9-IN-3 shows cell cytotoxicity and induces cell apoptosis. MMP-9-IN-3 can be used in the research of cancers[1].

  • CAS Number: 2581824-48-6
  • MF: C29H25N3O4
  • MW: 479.53
  • Catalog: Apoptosis
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

ICI 153110

ICI 153110 is an orally active phosphodiesterase inhibitor with both vasodilating and inotropic properties which is designed for the treatment of congestive cardiac failure.

  • CAS Number: 87164-90-7
  • MF: C11H11N3O
  • MW: 201.22500
  • Catalog: Phosphodiesterase (PDE)
  • Density: 1.22g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

UK122

UK122 is a potent and selective urokinase-type plasminogen activator (uPA) inhibitor with an IC50 of 0.2 μM. UK122 shows no or little inhibition of tissue-type PA (tPA), plasmin, thrombin, and trypsin (all IC50>100 μM). UK122, 4-oxazolidinone analogue, is an anticancer agent and inhibits cancer cell migration and invasion[1].

  • CAS Number: 940290-58-4
  • MF: C17H13N3O2
  • MW: 291.30
  • Catalog: Ser/Thr Protease
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Z-Gly-Pro-Arg-AMC · HCl

Z-Gly-Pro-Arg-AMC hydrochloride a fluorescent trypsin and cathepsin K substrate. Z-Gly-Pro-Arg-AMC hydrochloride can be used to determine trypsin and cathepsin K activity[1][2].

  • CAS Number: 201928-42-9
  • MF: C31H38ClN7O7
  • MW: 656.12900
  • Catalog: Cathepsin
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Teprenone

Teprenone is a anti-ulcer drug, and works as an inducer of heat shock proteins (HSPs).

  • CAS Number: 6809-52-5
  • MF: C23H38O
  • MW: 330.55
  • Catalog: HSP
  • Density: 0.9±0.1 g/cm3
  • Boiling Point: 442.2±24.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 168.0±9.8 °C

FITC-GW3965

FITC-GW3965 is a fluorescence-labelled liver X receptor β (LXRβ) agonist GW3965 (HY-10627). FITC-GW3965 is a tracer, that can be designed by replacing the trifluoromethyl of GW3965 with an amide to link the FITC. FITC-GW3965 can be used to study the function of LXRβ[1].

  • CAS Number: 2374144-23-5
  • MF: C59H56N4O9S
  • MW: 997.16
  • Catalog: LXR
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Aristololactam IIIa

Aristololactam IIIa exhibits significant inhibitory effects on superoxide anion generation and elastase release with IC50 values of 0.12 and 0.20 μg/mL, respectively[1].

  • CAS Number: 97399-89-8
  • MF: C16H9NO4
  • MW: 279.25
  • Catalog: Elastase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Patomostat mesilate

Patamostat (E-3123) mesylate is a potent protease inhibitor. Patamostat mesylate potently inhibits trypsin, plasmin and thrombin with IC50s of 39 nM, 950 nM and 1.9 μM, respectively. Patamostat mesylate may possess suppressing effects on pathogenesis and development of acute pancreatitis[1][2].

  • CAS Number: 114568-32-0
  • MF: C21H24N4O7S2
  • MW: 508.56800
  • Catalog: Ser/Thr Protease
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

SB-3CT

SB-3CT is a potent and competitive inhibitor of matrix metalloproteinase (MMP-2) and MMP-9.

  • CAS Number: 292605-14-2
  • MF: C15H14O3S2
  • MW: 306.400
  • Catalog: MMP
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 501.4±46.0 °C at 760 mmHg
  • Melting Point: 101 °C
  • Flash Point: 257.1±29.0 °C

BI 653048 phosphate

An oral glucocorticoid agonist for the treatment of rheumatoid arthritis. Rheumatoid Arthritis Phase 1 Clinical

  • CAS Number: 1198784-97-2
  • MF: C23H28F4N3O8PS
  • MW: 613.517
  • Catalog: Glucocorticoid Receptor
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Imsamotide

Imsamotide (IDO194-214) is a Indoleamine 2,3-Dioxygenase (IDO) peptide with sequences of DTLLKALLEIASCLEKALQVF, the IDO194-214. Imsamotide is also an immunological agent for active immunization, as well as an antineoplastic agent[1][2].

  • CAS Number: 2130836-27-8
  • MF: C106H180N24O31S
  • MW: 2318.76
  • Catalog: Indoleamine 2,3-Dioxygenase (IDO)
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

FTase-IN-1

FTase-IN-1 (compound 17a) is a potent and specific inhibitor of fanesyl transferase (FTase) with an IC50 of 0.35 μM. FTase-IN-1 displays cytotoxicity potential and antitumor activity[1].

  • CAS Number: 2490538-41-3
  • MF: C23H16N2O2S
  • MW: 384.45
  • Catalog: Farnesyl Transferase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Raltegravir sodium

Raltegravir (MK 0518) sodium is a potent and orally active integrase (IN) inhibitor, used to treat HIV infection.

  • CAS Number: 1292804-07-9
  • MF: C20H20FN6NaO5
  • MW: 466.40
  • Catalog: HIV
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Galegenimab

Galegenimab (FHTR 2163; RG 6147; RO 7171009), an anti-High-temperature requirement A1 (HTRA1) antibody fragment. Galegenimab can be used for age-related macular degeneration (AMD) research[1].

  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

(2S,3aR,7aS)-1-[(2S)-2-[[(2S)-1-ethoxy-1-oxo-4-phenylbutan-2-yl]amino]propanoyl]-2,3,3a,4,5,6,7,7a-octahydroindole-2-carboxylic acid,hydrochloride

Trandolapril (RU44570) hydrochloride is a nonsulfhydryl prodrug that is hydrolysed to the active diacid Trandolapril hydrochlorideat. Trandolapril hydrochloride is an orally active angiotensin converting enzyme (ACE) inhibitor that has been used in the treatment of hypertension and congestive heart failure (CHF), and after myocardial infarction (MI)[1].

  • CAS Number: 87725-72-2
  • MF: C24H35ClN2O5
  • MW: 466.99800
  • Catalog: Angiotensin-converting Enzyme (ACE)
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Deltasonamide 2 (TFA)

Deltasonamide 2 TFA is a PDEδ inhibitor with a Kd of ~385 pM and an EC50 of 1.24 μM[1].

  • CAS Number: 2235358-74-2
  • MF: C32H40ClF3N6O6S2
  • MW: 761.27
  • Catalog: Phosphodiesterase (PDE)
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

α-NETA

α-NETA is a potent and noncompetitive choline acetyltransferase (ChA) inhibitor with an IC50 of 9 μM. α-NETA is a potent ALDH1A1 (IC50=0.04 µM) and chemokine-like receptor-1 (CMKLR1) antagonist. α-NETA weakly inhibits cholinesterase (ChE; IC50=84 µM) and acetylcholinesterase (AChE; IC50=300 µM). α-NETA has anti-cancer activity[1][2].

  • CAS Number: 115066-04-1
  • MF: C16H20INO
  • MW: 369.25
  • Catalog: Apoptosis
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Palmitic acid-1,2,3,4-13C4

Palmitic acid-1,2,3,4-13C4 is the 13C-labeled Palmitic acid. Palmitic acid is a long-chain saturated fatty acid commonly found in both animals and plants. Palmitic acid can induce the expression of glucose-regulated protein 78 (GRP78) and CCAAT/enhancer binding protein homologous protein (CHOP) in in mouse granulosa cells[1][2].

  • CAS Number: 287100-89-4
  • MF: C1213C4H32O2
  • MW: 260.395
  • Catalog: HSP
  • Density: 0.9±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: 61-64 °C(lit.)
  • Flash Point: N/A

7-BIA

7-BIA is a receptor-type protein tyrosine phosphatase D (PTPRD) inhibitor with an IC50 of ~1-3 μM[1].

  • CAS Number: 1313403-49-4
  • MF: C15H18O6
  • MW: 294.30
  • Catalog: Phosphatase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A