MAPK families play an important role in complex cellular programs like proliferation, differentiation, development, transformation, and apoptosis. In mammalian cells, three MAPK families have been clearly characterized: namely classical MAPK (ERK), C-Jun N-terminal kinse/ stress-activated protein kinase (JNK/SAPK) and p38 kinase. Each MAPK-related cascade consists of no fewer than three enzymes that are activated in series: a MAPK kinase kinase (MAPKKK), a MAPK kinase (MAPKK) and a MAP kinase (MAPK).

The MAPK pathways are activated by diverse extracellular and intracellular stimuli including peptide growth factors, cytokines, hormones, and various cellular stressors. In the ERK signaling pathway, ERK1/2 is activated by MEK1/2, which is activated by Raf. Raf is activated by the Ras GTPase, whose activation is induced by RTKs such as the epidermal growth factor receptor. The JNK and p38 MAPK signaling pathways are activated by various types of cellular stress. The JNK pathway consists of JNK, a MAP2K such as MKK4 (SEK1) or MKK7, and a MAP3K such as ASK1, TAK1, MEKK1, or MLK3. In the p38 pathway, p38 is activated by MKK3 or MKK6, and these MAP2Ks are activated by the same MAP3Ks that function in the JNK pathway.

MAPK signaling pathways has been implicated in the development of many human diseases including Alzheimer's disease (AD), Parkinson's disease (PD), amyotrophic lateral sclerosis (ALS) and various types of cancers. Therefore, the development of small molecule drugs that selectively inhibit individual components of MAPK signaling pathways is a key therapeutic strategy for cancer and neurodegenerative disorders.

References:
[1] Zhang W, et al. Cell Research (2002) 12, 9-18.
[2] Kim EK, et al. Biochim Biophys Acta. 2010 Apr;1802(4):396-405.
[3] Kim EK, et al. Arch Toxicol. 2015 Jun;89(6):867-82.


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ETC-206

ETC-206 is a selective MNK1 and MNK2 inhibitor with IC50s of 64 nM and 86 nM, respectively.

  • CAS Number: 1464151-33-4
  • MF: C25H20N4O2
  • MW: 408.45
  • Catalog: MNK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

HPK1-IN-34

HPK1-IN-34 (Compound 143) is a Hematopoietic progenitor kinase 1 (HPK1) inhibitor with an IC50 of <100 nM[1].

  • CAS Number: 2380300-99-0
  • MF: C25H28N4O2S
  • MW: 448.58
  • Catalog: MAP4K
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

MLK-IN-1

MLK-IN-1 is a potent, brain penetrant and specific mixed lineage kinase 3 (MLK-3) inhibitor, compound 68, extracted from patent US20140256733A1[1].

  • CAS Number: 1627729-62-7
  • MF: C23H20N4O3S
  • MW: 432.49
  • Catalog: Mixed Lineage Kinase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

ML 3403

ML3403 is a potent p38 MAPK inhibitor with an IC50 of 0.38 μM[1].

  • CAS Number: 549505-65-9
  • MF: C23H21FN4S
  • MW: 404.50300
  • Catalog: p38 MAPK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

FR 167653

FR-167653 is a selective p38 MAPK inhibitor.

  • CAS Number: 158876-66-5
  • MF: C24H20FN5O6S
  • MW: 525.50900
  • Catalog: p38 MAPK
  • Density: N/A
  • Boiling Point: 724.4ºC at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 391.9ºC

SD-06

SD 0006 (SD-06) is a p38 MAP kinase inhibitor; inhibits p38α with an IC50 value of 170 nM and inhibits LPS-stimulated TNF-release in rats (83% inhibition at 1mg/kg, po).

  • CAS Number: 271576-80-8
  • MF: C20H20ClN5O2
  • MW: 397.858
  • Catalog: p38 MAPK
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: 652.6±55.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 348.5±31.5 °C

Edaxeterkib

Edaxeterkib is a potent extracellular signal-​regulated kinase (ERK) inhibitor for the research of cancer[1].

  • CAS Number: 1695534-88-3
  • MF: C26H27N7O2
  • MW: 469.54
  • Catalog: ERK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

TAT-MEK1

TAT-MEK1 is an inhibitor ofERK2, consisting of TAT and MEK1 (N-terminal), TAT (YGRKKRRQRRR) derived from human immunodeficiency (HIV-1) transcriptional trans activator (TAT), is a cell-penetrating peptide. TAT-MEK1 IC50 in vitro for ERK2 is 29 μM[1][2].

  • CAS Number: 566872-16-0
  • MF: C136H241N53O31S
  • MW: 3146.77
  • Catalog: ERK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

BIX 02565

BIX 02565 is a potent ribosomal S6 kinase 2 (RSK2) inhibitor with IC50 of 1.1 nM.

  • CAS Number: 1311367-27-7
  • MF: C26H30N6O2
  • MW: 458.55500
  • Catalog: Ribosomal S6 Kinase (RSK)
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

HPK1-IN-19

HPK1-IN-19 is a hematopoietic progenitor kinase 1 (HPK1) inhibitor extracted from patent WO2018102366A1 compound I-47[1].

  • CAS Number: 2227609-33-6
  • MF: C27H32N7O2P
  • MW: 517.56
  • Catalog: MAP4K
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Big Endothelin-1 (1-31) (human, bovine) trifluoroacetate salt

Endothelin-1 (1-31) (Human) is a potent vasoconstrictor and hypertensive agent. Endothelin-1 (1-31) (Human) is derived from the selective hydrolysis of big ET-1 by chymase[1].

  • CAS Number: 133972-52-8
  • MF: C162H236N38O47S5
  • MW: 3628.159
  • Catalog: ERK
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

FR 167653 free base

FR-167653 is a selective p38 MAPK inhibitor.

  • CAS Number: 158876-65-4
  • MF: C24H18FN5O2
  • MW: 427.43
  • Catalog: p38 MAPK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Piplartine

Piperlongumine is a natural alkaloid isolated from Piper longum Linn[1], possesses ant-inflammatory, antibacterial, antiangiogenic, antioxidant, antitumor, and antidiabetic activities[2]. Piperlongumine induces ROS, and induces apoptosis in cancer cell lines[1]. Piperlongumine shows anti-cardiac fibrosis activity, suppresses myofibroblast transformation via suppression of the ERK1/2 signaling pathway[2].

  • CAS Number: 20069-09-4
  • MF: C17H19NO5
  • MW: 317.336
  • Catalog: ERK
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 475.6±45.0 °C at 760 mmHg
  • Melting Point: 124ºC
  • Flash Point: 241.4±28.7 °C

OVA-E1 peptide TFA

OVA-E1 peptide TFA, is an antagonist variant of SIINFEKL [OVA (257-264). OVA-E1 peptide, activates the p38 and JNK cascades similarly in mutant and wild-type thymocytes[1].

  • CAS Number: 1262750-80-0
  • MF: C49H77F3N10O16
  • MW: 1119.19
  • Catalog: JNK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

3',4',7-trihydroxy isoflavone

7,3',4'-Trihydroxyisoflavone, a major metabolite of Daidzein, is an ATP-competitive inhibitor of Cot (Tpl2/MAP3K8) and MKK4. 7,3',4'-Trihydroxyisoflavone has anticancer, anti-angiogenic, chemoprotective, and free radical scavenging activities[1][2].

  • CAS Number: 485-63-2
  • MF: C15H10O5
  • MW: 270.24
  • Catalog: Apoptosis
  • Density: 1.5±0.1 g/cm3
  • Boiling Point: 572.8±50.0 °C at 760 mmHg
  • Melting Point: 280-282°C
  • Flash Point: 224.0±23.6 °C

ADTL-EI1712

ADTL-EI1712 is a potent, orally active, and selective dual-target inhibitor of ERK1 and ERK5, inhibition rates of ERK1/5 at 1 μM are 93.54% and 89.35%, respectively. ADTL-EI1712 can induce regulated cell death, a form of cell death that relies on the activation of genetically encoded machinery, to overcome compensatory mechanism in specific cancer cells in vitro and in vivo[1].

  • CAS Number: 2414916-45-1
  • MF: C22H18Cl2N4O2S2
  • MW: 505.440
  • Catalog: ERK
  • Density: 1.6±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

STE-MEK1(13)

STE-MEK1(13) (Ste-MPKKKPTPIQLNP-NH?) is a cell permeable ERK1/2 inhibitor (IC50: 13-30?μM). STE-MEK1(13) inhibits ERK1/2 phosphorylation[1][2].

  • CAS Number: 566872-15-9
  • MF: C86H153N19O17S
  • MW: 1757.32
  • Catalog: ERK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

H-Lys-Arg-Glu-Leu-Val-Glu-Pro-Leu-Thr-Pro-Ser-Gly-Glu-Ala-Pro-Asn-Gln-Ala-Leu-Leu-Arg-OH

EGF-R (661-681) T669 Peptide is a MAPK substrate that can used to measure MAPK catalytic activity[1].

  • CAS Number: 137348-21-1
  • MF: C101H172N30O32
  • MW: 1475.645
  • Catalog: p38 MAPK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

RSK2-IN-3

RSK2-IN-3 (Compound 26) is a reversible covalent inhibitor of the RPS6KA3 (RSK2) kinase[1].

  • CAS Number: 1627136-54-2
  • MF: C24H26N4O5
  • MW: 450.49
  • Catalog: Ribosomal S6 Kinase (RSK)
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

JWG-071

JWG-071 is the first reported kinase-selective chemical probe for ERK5. JWG-071 improves ERK5 activity and BRD4 selectivity. JWG-071 will be a much-needed chemical probe for deconvoluting ERK5 and BRD4 pharmacology[1].

  • CAS Number: 2250323-50-1
  • MF: C34H44N8O3
  • MW: 612.76
  • Catalog: ERK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Ginsenoside Re

Ginsenoside Re is an extract from Panax notoginseng. Ginsenoside Re decreases the β-amyloid protein (Aβ). Ginsenoside Re plays a role in antiinflammation through inhibition of JNK and NF-κB.

  • CAS Number: 52286-59-6
  • MF: C48H82O18
  • MW: 947.154
  • Catalog: JNK
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: 1011.8±65.0 °C at 760 mmHg
  • Melting Point: 202 °C(dec.)
  • Flash Point: 565.7±34.3 °C

CHMFL-EGFR-202

CHMFL-EGFR-202 is a potent, irreversible inhibitor of epidermal growth factor receptor (EGFR) mutant kinase, with IC50s of 5.3 nM and 8.3 nM for drug-resistant mutant EGFR T790M and WT EGFR kinases, respectively. CHMFL-EGFR-202 exhibits ∼10-fold selectivity for EGFR L858R/T790M against the EGFR wild-type in cells. CHMFL-EGFR-202 adopts a covalent “DFG-in-C-helix-out” inactive binding conformation with EGFR, with strong antiproliferative effects against EGFR mutant-driven nonsmall-cell lung cancer (NSCLC) cell lines[1].

  • CAS Number: 2089381-40-6
  • MF: C25H24ClN7O2
  • MW: 489.96
  • Catalog: EGFR
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Trametinib (DMSO solvate)

Trametinib DMSO solvate is a potent MEK inhibitor that specifically inhibits MEK1/2, with an IC50 value of about 2 nM.

  • CAS Number: 1187431-43-1
  • MF: C28H29FIN5O5S
  • MW: 693.528
  • Catalog: MEK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Hirsutenone

Hirsutenone is an active botanical diarylheptanoid present in Alnus species and exhibits many biological activities, including anti-inflammatory, anti-tumor promoting and anti-atopic dermatitis effects. Hirsutenone attenuates adipogenesis by binding directly to PI3K and ERK1 in a non-ATP competitive manner. Hirsutenone can be used for the study of obesity[1].

  • CAS Number: 41137-87-5
  • MF: C19H20O5
  • MW: 328.359
  • Catalog: ERK
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 622.0±55.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 344.0±28.0 °C

HPK1-IN-11

HPK1-IN-11 is potent inhibitor of HPK1. HPK1 is a serine/threonine protein kinase cloned from hematopoietic progenitor cells and belongs to the MAP4K family of mammalian Ste-20-related protein kinases. HPK1-IN-11 has the potential for the research of HPK1 related diseases (extracted from patent WO2021213317A1, compound 2)[1].

  • CAS Number: 2734167-68-9
  • MF: C27H25N5O2
  • MW: 451.52
  • Catalog: MAP4K
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

p38 MAP Kinase Inhibitor IV

p38 MAP Kinase Inhibitor IV is a highly specific ATP-competitive p38α MAPK inhibitor with IC50s of 0.13 and 0.55 μM for p38α and p38β MAPK, respectively[1].

  • CAS Number: 1638-41-1
  • MF: C12H4Cl6O4S
  • MW: 456.94100
  • Catalog: p38 MAPK
  • Density: 1.854 g/cm3
  • Boiling Point: 590ºC at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 310.6ºC

Selumetinib (AZD6244)

Selumetinib is a highly potent MEK inhibitor, with an IC50 of 14 nM against MEK1.

  • CAS Number: 606143-52-6
  • MF: C17H15BrClFN4O3
  • MW: 457.681
  • Catalog: MEK
  • Density: 1.7±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

ERK Inhibitor II (Negative control)

ERK Inhibitor II (Negative control) is an effective inhibitor of extracellular signal-regulated kinase (ERK). ERK Inhibitor II (Negative control) inhibits the activation of insulin receptor, which can be used in the study of diabetes[1][2].

  • CAS Number: 1177970-73-8
  • MF: C18H12N6O
  • MW: 328.32700
  • Catalog: ERK
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

FR 180204

FR 180204 is an ATP-competitive, selective ERK inhibitor with Ki of 0.31 μM and 0.14 μM for ERK1 and ERK2, respectively.

  • CAS Number: 865362-74-9
  • MF: C18H13N7
  • MW: 327.343
  • Catalog: ERK
  • Density: 1.6±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Sesamolin

Sesaminol, isolated from Justicia orbiculata, has antioxidative activity, Sesaminol inhibits lipid peroxidation and shows neuroprotection effect. Sesaminol potently inhibits MAPK cascades by preventing phosphorylation of JNK, p38 MAPKs, and caspase-3 but not ERK-MAPK expression[1][2][3][4].

  • CAS Number: 526-07-8
  • MF: C20H18O7
  • MW: 370.353
  • Catalog: Caspase
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: 520.8±50.0 °C at 760 mmHg
  • Melting Point: 93 - 94ºC (Decomposes)
  • Flash Point: 219.2±30.0 °C