Metabolic pathways are enzyme-mediated biochemical reactions that lead to biosynthesis (anabolism) or breakdown (catabolism) of natural product small molecules within a cell or tissue. In each pathway, enzymes catalyze the conversion of substrates into structurally similar products. Metabolic processes typically transform small molecules, but also include macromolecular processes such as DNA repair and replication, and protein synthesis and degradation. Metabolism maintains the living state of the cells and the organism.

Proteases are used throughout an organism for various metabolic processes. Proteases control a great variety of physiological processes that are critical for life, including the immune response, cell cycle, cell death, wound healing, food digestion, and protein and organelle recycling. On the basis of the type of the key amino acid in the active site of the protease and the mechanism of peptide bond cleavage, proteases can be classified into six groups: cysteine, serine, threonine, glutamic acid, aspartate proteases, as well as matrix metalloproteases. Proteases can not only activate proteins such as cytokines, or inactivate them such as numerous repair proteins during apoptosis, but also expose cryptic sites, such as occurs with β-secretase during amyloid precursor protein processing, shed various transmembrane proteins such as occurs with metalloproteases and cysteine proteases, or convert receptor agonists into antagonists and vice versa such as chemokine conversions carried out by metalloproteases, dipeptidyl peptidase IV and some cathepsins. In addition to the catalytic domains, a great number of proteases contain numerous additional domains or modules that substantially increase the complexity of their functions.

Imbalances in metabolic activities have been found to be critical in a number of pathologies, such as cardiovascular diseases, inflammation, cancer, and neurodegenerative diseases.

References:
[1] Turk B, et al. EMBO J. 2012 Apr 4;31(7):1630-43.
[2] Eatemadi A, et al. Biomed Pharmacother. 2017 Feb;86:221-231.


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SHP836

SHP836 is a distinct, allosteric site 2 SHP2 inhibitor with IC50 of 12 uM (SHP2 1-525), and selective over the phosphatase domain (SHP2 PTP IC50>100 uM).downregulates DUSP6 mRNA, a downstream MAPK pathway marker, in KYSE-520 cancer cells; enhances pharmacological pathway inhibition in cells combined with SHP099.

  • CAS Number: 1957276-35-5
  • MF: C16H19Cl2N5
  • MW: 352.263
  • Catalog: Phosphatase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

GYKI 47261 dihydrochloride

GYKI-47261 dihydrochloride is a competitive, orally active, and selective AMPA receptor antagonist with an IC50 of 2.5 μM. GYKI-47261 has broad spectrum anticonvulsive activity and neuroprotective effects. GYKI-47261 dihydrochloride is also a potent inducer of CYP2E1[1][2].

  • CAS Number: 1217049-32-5
  • MF: C18H17Cl3N4
  • MW: 395.713
  • Catalog: iGluR
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Calcineurin autoinhibitory peptide

Calcineurin autoinhibitory peptide is a selective inhibitor of Ca2+/calmodulin-dependent protein phosphatase (calcineurin), with an IC50 of ~10 μM. Calcineurin autoinhibitory peptide could protect neurons from excitatory neuronal death[1][2].

  • CAS Number: 148067-21-4
  • MF: C124H205N39O39S2
  • MW: 2930.32000
  • Catalog: Phosphatase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

VT-464 (racemate)

Seviteronel (VT-464) racemate is the racemate form of Seviteronel (VT-464), which is a potent CYP17 lyase inhibitor(h-Lyase IC50=nM)inhibition.

  • CAS Number: 1375603-36-3
  • MF: C18H17F4N3O3
  • MW: 399.33900
  • Catalog: Cytochrome P450
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

2'-Ethyl Simvastatin

2'-Ethyl Simvastatin (compound 6) is a Mevinolin analog, with HMG-CoA reductase inhibition[1].

  • CAS Number: 79902-42-4
  • MF: C23H34O5
  • MW: 390.51300
  • Catalog: HMG-CoA Reductase (HMGCR)
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

ROR gamma-t-IN-1

GSK805 is a potent, orally bioavailable RORγγ Inhibitor with pIC50 of 8.4 and >8.2 for RORγ FRET assay and Th17 assay.

  • CAS Number: 1426802-50-7
  • MF: C23H18Cl2F3NO4S
  • MW: 532.359
  • Catalog: ROR
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: 617.1±55.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 327.0±31.5 °C

RORγt Inverse agonist 2

RORγt Inverse agonist 2 is a selective, orally active RORγt inverse agonist with an EC50 of 119 nM[1].

  • CAS Number: 1801197-92-1
  • MF: C27H25F8NO5S
  • MW: 627.54
  • Catalog: ROR
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

4-Biphenylac-Cys(Me)-D-Arg-Phe-(2-phenylethyl)amide

Cathepsin L-IN-3 is a tripeptide-sized cathepsin L inhibitor.

  • CAS Number: 478164-48-6
  • MF: C41H49N7O4S
  • MW: 735.93700
  • Catalog: Cathepsin
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Falcarinol

Falcarinol (Panaxynol) is a natural, orally active Hsp90 inhibitor targeting both the N-terminal and C-terminal of Hsp90 with limited toxicities. Falcarinol (Panaxynol) induces apoptosis[1].

  • CAS Number: 21852-80-2
  • MF: C17H24O
  • MW: 244.372
  • Catalog: HSP
  • Density: 0.9±0.1 g/cm3
  • Boiling Point: 370.6±42.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 163.4±20.9 °C

Dabigatran etexilate

Dabigatran etexilate(BIBR-1048) is the orally active prodrug of dabigatran; Dabigatran is a reversible and selective, direct thrombin inhibitor (DTI) with Ki value of 4.5 nM.IC50 Value: 4.5 nM (Ki); 10 nM(Thrombin-induced platelet aggregation) [1]in vitro: Dabigatran selectively and reversibly inhibited human thrombin(Ki: 4.5 nM) as well as thrombin-induced platelet aggregation (IC(50): 10 nM), while showing no inhibitory effect on other platelet-stimulating agents.Thrombin generation in platelet-poor plasma (PPP), measured as the endogenous thrombin potential (ETP) was inhibited concentration-dependently (IC(50): 0.56 microM). Dabigatran demonstrated concentration-dependent anticoagulant effects in various species in vitro, doubling the activated partial thromboplastin time (aPTT), prothrombin time (PT) and ecarin clotting time (ECT) in human PPP at concentrations of 0.23, 0.83 and 0.18 microM, respectively [1]. in vivo: Dabigatran prolonged the aPTT dose-dependently after intravenous administration in rats (0.3, 1 and 3 mg/kg) and rhesus monkeys (0.15, 0.3 and 0.6 mg/kg). Dose- and time-dependent anticoagulant effects were observed with dabigatran etexilate administered orally to conscious rats (10, 20 and 50 mg/kg) or rhesus monkeys (1, 2.5 or 5 mg/kg), with maximum effects observed between 30 and 120 min after administration, respectively [1]. Patients treated with dabigatran etexilate experienced fewer ischaemic strokes (3.74 dabigatran etexilate vs 3.97 warfarin) and fewer combined intracranial haemorrhages and haemorrhagic strokes (0.43 dabigatran etexilate vs 0.99 warfarin) per 100 patient-years [2].Clinical trial: An Evaluation of the Pharmacokinetics and Pharmacodynamics of Oral Dabigatran Etexilate in Hemodialysis Patients . Phase1

  • CAS Number: 211915-06-9
  • MF: C34H41N7O5
  • MW: 627.733
  • Catalog: Thrombin
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 827.9±75.0 °C at 760 mmHg
  • Melting Point: 128-129°
  • Flash Point: 454.5±37.1 °C

D-Glucosamine hydrochloride

Glucosamine (hydrochloride) is a natural product.IC50 value:Target:In vitro: Glucosamine hydrochloride exhibited dose-dependent DPPH antioxidant activity [1]. Short-term (4 h) glucosamine hydrochloride treatment inhibited HIF-1α at the protein level, decreased phosphorylation of p70S6K and S6, translation-related proteins [2]. In the obstructed kidneys and TGF-β1-treated renal cells, glucosamine hydrochloride significantly decreased renal expression of α-smooth muscle actin, collagen I, and fibronectin [3]. In vivo:

  • CAS Number: 66-84-2
  • MF: C6H14ClNO5
  • MW: 215.632
  • Catalog: Autophagy
  • Density: N/A
  • Boiling Point: 449.9ºC at 760 mmHg
  • Melting Point: 190-194ºC
  • Flash Point: 225.9ºC

INT-767

INT-767 is a dual farnesoid X receptor/TGR5 agonist with mean EC50s of 30 and 630 nM, respectively.

  • CAS Number: 1000403-03-1
  • MF: C25H43NaO6S
  • MW: 494.66
  • Catalog: GPCR19
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

CETP-IN-3

CETP-IN-3 (Compound 13) is an small molecule inhibitor of the plasma glycoprotein cholesterol ester transfer protein (CETP), elevating HDL-C through inhibition of CETP. CETP-IN-3 for the CETP inhibitory activity in the scintillation proximity (SPA) and whole plasma assay (WPA) with IC50s of 0.002 μM and 0.06 μM, respectively[1].

  • CAS Number: 939391-31-8
  • MF: C30H24F12N2O4
  • MW: 704.50
  • Catalog: CETP
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

AAA-pNA

AAA-pNA is a chromogenic substrate of Tripeptidyl-peptidase II. AAA-pNA can be used to test Tripeptidyl-peptidase II activity[1].

  • CAS Number: 60354-61-2
  • MF: C15H21N5O5
  • MW: 351.36
  • Catalog: Ser/Thr Protease
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Veledimex (S enantiomer)

Veledimex S enantiomer is the S enantiomer of veledimex. Veledimex is an oral activator ligand for a proprietary gene therapy promoter system, and a moderate inhibitor of and substrate for CYP3A4/5.

  • CAS Number: 1093131-03-3
  • MF: C27H38N2O3
  • MW: 438.6
  • Catalog: Interleukin Related
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

COX/5-LO-IN-1

COX/5-LO-IN-1 is an inhibitor of cylooxygenase and 5-lipoxygenase, used for the research of inflammatory and allergic disease states.

  • CAS Number: 154355-75-6
  • MF: C16H15FN2O2S
  • MW: 318.371
  • Catalog: 5-Lipoxygenase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Lycopodine

Lycopodine, a pharmacologically important bioactive component derived from Lycopodium clavatumspores, triggers apoptosis by modulating 5-lipoxygenase, and depolarizing mitochondrial membrane potential in refractory prostate cancer cells without modulating p53 activity[1]. Lycopodine inhibits proliferation of HeLa cells through induction of apoptosis via caspase-3 activation[2].

  • CAS Number: 466-61-5
  • MF: C16H25NO
  • MW: 247.37600
  • Catalog: Caspase
  • Density: 1.106g/cm3
  • Boiling Point: 373.464ºC at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 137.245ºC

CMS121

CMS-121 is a quinolone derivative and an orally active acetyl-CoA carboxylase 1 (ACC1) inhibitor. CMS-121 protects HT22 cells against ischemia and oxidative damage with EC50 values of 7 nM and 200 nM, respectively. CMS-121 has strong neuroprotective, anti-inflammatory, antioxidative and renoprotective activities[1][2][3].

  • CAS Number: 1353224-53-9
  • MF: C20H19NO3
  • MW: 321.37
  • Catalog: Acetyl-CoA Carboxylase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

(-)-PX20606 trans isomer

(-)-PX20606 trans isomer is a FXR agonist with EC50s of 18 and 29 nM for FXR in FRET and M1H assay, respectively.

  • CAS Number: 1268244-88-7
  • MF: C29H22Cl3NO4
  • MW: 553.06
  • Catalog: FXR
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

E6446

E6446 is a potent and orally acitve TLR7 and TLR9 antagonist, used in the research of deleterious inflammatory responses.

  • CAS Number: 1219925-73-1
  • MF: C27H35N3O3
  • MW: 449.58500
  • Catalog: Toll-like Receptor (TLR)
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Tetrapeptide-30

Tetrapeptide-30 is a skin-brightening peptide consisting of four amino acids. Tetrapeptide-30 can act as a tyrosinase inhibitor, lightening hyperpigmentation and evening out skin tone by reducing the amount of tyrosinase and inhibiting melanocyte activation[1].

  • CAS Number: 1036207-61-0
  • MF: C22H40N6O7
  • MW: 500.596
  • Catalog: Tyrosinase
  • Density: 1.256±0.06 g/cm3(Predicted)
  • Boiling Point: 931.5±65.0 °C(Predicted)
  • Melting Point: N/A
  • Flash Point: N/A

AMG-1694

AMG-1694 is a potent glucokinase–glucokinase regulatory protein (GK-GKRP) disruptors and promotes the dissociation of the GK-GKRP complex with an IC50 of 7 nM, indirectly increasing GK enzymatic activity. AMG-1694 potently reverses the inhibitory effect of GKRP on GK activity and promotes GK translocation. AMG-1694 normalizes blood glucose levels in several rodent models of diabetes and lowes blood glucose restricted to diabetic and not normoglycaemic animals[1].

  • CAS Number: 1361217-07-3
  • MF: C23H30F3N3O4S2
  • MW: 533.63
  • Catalog: Glucokinase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Raltegravir (MK-0518)

Raltegravir is a potent integrase (IN) inhibitor, used to treat HIV infection.

  • CAS Number: 518048-05-0
  • MF: C20H21FN6O5
  • MW: 444.416
  • Catalog: HIV
  • Density: 1.5±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Vatiquinone

Vatiquinone is a potent cellular oxidative stress protectant, which could be used to treat mitochondrial diseases.

  • CAS Number: 1213269-98-7
  • MF: C29H44O3
  • MW: 440.658
  • Catalog: Mitochondrial Metabolism
  • Density: 1.0±0.1 g/cm3
  • Boiling Point: 565.6±39.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 309.9±23.6 °C

Rentiapril racemate

Rentiapril racemate is the racemate of Rentiapril. Rentiapril is an angiotensin converting enzyme (ACE) inhibitor.

  • CAS Number: 72679-47-1
  • MF: C13H15NO4S2
  • MW: 313.39300
  • Catalog: Angiotensin-converting Enzyme (ACE)
  • Density: 1.451g/cm3
  • Boiling Point: 553.7ºC at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 288.7ºC

PDE5/HDAC-IN-1

PDE5/HDAC-IN-1 (Compound 26) is a potent phosphodiesterase 5 (PDE5) and HDAC inhibitor with IC50 values of 46.3 nM and 14.5 nM, respectively. PDE5/HDAC-IN-1 induces cell apoptosis and shows anticancer activities[1].

  • CAS Number: 2414921-48-3
  • MF: C27H29BrN4O4
  • MW: 553.45
  • Catalog: Apoptosis
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Adjudin

Adjudin is an extensively studied male contraceptive with a superior mitochondria-inhibitory effect. Adjudin is also a potent Cl- channel blocker.

  • CAS Number: 252025-52-8
  • MF: C15H12Cl2N4O
  • MW: 335.188
  • Catalog: Chloride Channel
  • Density: 1.5±0.1 g/cm3
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Polmacoxib

Polmacoxib (CG100649) is a first-in-class, orally active nonsteroidal anti-inflammatory drug (NSAID) which is a dual inhibitor of COX-2 (IC50 around 0.1 μg/ml) and carbonic anhydrase[1]. Polmacoxib inhibits colorectal adenoma and tumor growth in mouse models[2].

  • CAS Number: 301692-76-2
  • MF: C18H16FNO4S
  • MW: 361.38700
  • Catalog: COX
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

SA 57

SA57 is a potent, selective FAAH inhibitor with IC50s of 3.2 nM and 1.9 nM for mouse and human FAAH. SA57 also inhibits the 2-arachidonoylglycerol hydrolases MAGL (IC50s of 410 nM and 1.4 μM for mouse and human MAGL) and mouse α/β-hydrolase domain-containing protein 6 (mABHD6; IC50 of 850 nM), but not other brain serine hydrolases[1][2].

  • CAS Number: 1346169-63-8
  • MF: C17H23ClN2O3
  • MW: 338.829
  • Catalog: FAAH
  • Density: 1.2±0.1 g/cm3
  • Boiling Point: 512.1±30.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 263.5±24.6 °C

PD 113413

PD 113413 is formed by subsequent hydrolysis of the diketopiperazine quinapril analog. PD 113413 is a potent angiotensin-converting enzyme inhibitor. PD 113413 can be used for research of hypertension and congestive heart failure[1].

  • CAS Number: 103733-50-2
  • MF: C23H24N2O4
  • MW: 392.45
  • Catalog: Angiotensin-converting Enzyme (ACE)
  • Density: 1.33g/cm3
  • Boiling Point: 669.8ºC at 760mmHg
  • Melting Point: N/A
  • Flash Point: 358.9ºC