Topoisomerases are enzymes that regulate the overwinding or underwinding of DNA. The winding problem of DNA arises due to the intertwined nature of its double-helical structure. Topoisomerases are isomerase enzymes that act on the topology of DNA. Type I topoisomerase cuts one strand of a DNA double helix, relaxation occurs, and then the cut strand is reannealed. Type I topoisomerases are subdivided into two subclasses: type IA topoisomerases, which share many structural and mechanistic features with the type II topoisomerases, and type IB topoisomerases, which utilize a controlled rotary mechanism. Type II topoisomerase cuts both strands of one DNA double helix, pass another unbroken DNA helix through it, and then reanneal the cut strands. This class is also split into two subclasses: type IIA and type IIB topoisomerases, which possess similar structure and mechanisms.


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Topoisomerase I inhibitor 3

Topoisomerase I inhibitor 3 (Compound ZML-14) is a topoisomerase I inhibitor and can interact with topoisomerase I-DNA complex. Topoisomerase I inhibitor 3 induces HepG2 cell apoptosis and arrests cell cycle at G2/M phase[1].

  • CAS Number: 2588211-50-9
  • MF: C18H14FNO3
  • MW: 311.31
  • Catalog: Apoptosis
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

7-Ethyl-10-hydroxycamptothecin

SN-38 is an active metabolite of the Topoisomerase I inhibitor Irinotecan. SN-38 inhibits DNA and RNA synthesis with IC50s of 0.077 and 1.3 μM, respectively.

  • CAS Number: 86639-52-3
  • MF: C22H20N2O5
  • MW: 392.405
  • Catalog: Autophagy
  • Density: 1.5±0.1 g/cm3
  • Boiling Point: 810.3±65.0 °C at 760 mmHg
  • Melting Point: 217 °C
  • Flash Point: 443.8±34.3 °C

Trovafloxacin Mesylate

Trovafloxacin mesylate is a broad-spectrum quinolone antibiotic with potent activity against Gram-positive, Gram-negative and anaerobic organisms. Trovafloxacin mesylate blocks the DNA gyrase and topoisomerase IV activity. Trovafloxacin mesylate is also a potent, selective and orally active pannexin 1 channel (PANX1) inhibitor with an IC50 of 4 μM for PANX1 inward current. Trovafloxacin mesylate does not inhibit connexin 43 gap junction or PANX2. Trovafloxacin mesylate leads to dysregulated fragmentation of apoptotic cells by inhibiting PANX1[1][2][3].

  • CAS Number: 147059-75-4
  • MF: C21H19F3N4O6S
  • MW: 512.45900
  • Catalog: Bacterial
  • Density: N/A
  • Boiling Point: 630.5ºC at 760mmHg
  • Melting Point: >250 °C (dec.)
  • Flash Point: 335.1ºC

Mitoxantrone

Mitoxantrone is a topoisomerase II inhibitor; also inhibits protein kinase C (PKC) activity with an IC50 of 8.5 μM.

  • CAS Number: 65271-80-9
  • MF: C22H28N4O6
  • MW: 444.481
  • Catalog: Topoisomerase
  • Density: 1.5±0.1 g/cm3
  • Boiling Point: 805.7±65.0 °C at 760 mmHg
  • Melting Point: 170-174ºC
  • Flash Point: 441.1±34.3 °C

9-Chloromethyl-10-hydroxy-11-F-Camptothecin

9-Chloromethyl-10-hydroxy-11-F-Camptothecin is a novel camptothecin derivatives.9-Chloromethyl-10-hydroxy-11-F-Camptothecin is a DNA topoisomerase I (Topo I) inhibitor, can be used in anticancer research[1].

  • CAS Number: 2414594-22-0
  • MF: C22H18ClFN2O4
  • MW: 428.84
  • Catalog: Topoisomerase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Exatecan mesylate

Exatecan (DX-8951) mesylate dihydrate is a DNA topoisomerase I inhibitor, with an IC50 of 2.2 μM (0.975 μg/mL), and can be used in cancer research[1][2][3].

  • CAS Number: 197720-53-9
  • MF: C24H22FN3O4S.CH4O3S.2H2O
  • MW: 567.58
  • Catalog: ADC Cytotoxin
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Trovafloxacin-d4 mesylate

Trovafloxacin-d4 mesylate is the deuterium labeled Trovafloxacin mesylate. Trovafloxacin mesylate is a broad-spectrum quinolone antibiotic with potent activity against Gram-positive, Gram-negative and anaerobic organisms. Trovafloxacin mesylate blocks the DNA gyrase and topoisomerase IV activity. Trovafloxacin mesylate is also a potent, selective and orally active pannexin 1 channel (PANX1) inhibitor with an IC50 of 4 μM for PANX1 inward current. Trovafloxacin mesylate does not inhibit connexin 43 gap junction or PANX2. Trovafloxacin mesylate leads to dysregulated fragmentation of apoptotic cells by inhibiting PANX1[1][2][3].

  • CAS Number: 1346601-60-2
  • MF: C21H15D4F3N4O6S
  • MW: 516.48
  • Catalog: Bacterial
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Br-Val-Ala-NH2-bicyclo[1.1.1]pentane-7-MAD-MDCPT

Br-Val-Ala-NH2-bicyclo[1.1.1]pentane-7-MAD-MDCPT (Formula V) is a drug-linker conjugate that composed of a potent topoisomerase I inhibitor and a linker to make antibody drug conjugate (ADC)[1].

  • CAS Number: 2857037-70-6
  • MF: C36H38BrN5O9
  • MW: 764.62
  • Catalog: Topoisomerase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

NBTIs-IN-6

NBTIs-IN-6 is a bacterial topoisomerase inhibitor with certain antibacterial activity. Among them, the MIC90 for fluoroquinolone-resistant MRSA was 2 μg/mL[1].

  • CAS Number: 2422893-97-6
  • MF: C25H27FN4O6
  • MW: 498.50
  • Catalog: Topoisomerase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Amrubicin

Amrubicin is a DNA topoisomerase II inhibitor, used for the research of cancer.

  • CAS Number: 110267-81-7
  • MF: C25H25NO9
  • MW: 483.467
  • Catalog: Topoisomerase
  • Density: 1.6±0.1 g/cm3
  • Boiling Point: 717.8±60.0 °C at 760 mmHg
  • Melting Point: 172-174ºC
  • Flash Point: 387.9±32.9 °C

3,6-Diamino-9(10H)-acridone

3,6-Diamino-9(10H)-acridone is a topoisomerase inhibitor[1].

  • CAS Number: 42832-87-1
  • MF: C13H11N3O
  • MW: 225.24600
  • Catalog: Topoisomerase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: >300ºC dec.
  • Flash Point: N/A

CH-0793076

CH-0793076 (TP3076), a hexacyclic camptothecin analog, is active drug and major metabolite of TP300. CH-0793076 inhibits DNA topoisomerase I with an IC50 of 2.3 μM. CH-0793076 is efficacious against cells expressing BCRP (breast cancer resistance protein)[1].

  • CAS Number: 534605-78-2
  • MF: C26H26N4O4
  • MW: 458.51
  • Catalog: Topoisomerase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Podocarpusflavone A

Podocarpusflavone A is a DNA topoisomerase I inhibitor, have moderated anti-proliferative activity induce cell apoptosis in MCF-7, is developing anti-tumor drugstarget: DNA topoisomerase IIn vitro: podocarpusflavone-A show significant inhibitions against DLD, KB, MCF-7, HEp-2 tumor cell lines (ED50 4.56-16.24 μg/mL) and induce cell apoptosis in MCF-7 via mainly sub-G1/S phase arrest. PF (40 ug/mL, 24 hr) significantly induced about 10 folds of cell deaths and growth arrest in S-phase than the control group.

  • CAS Number: 22136-74-9
  • MF: C31H20O10
  • MW: 552.484
  • Catalog: Apoptosis
  • Density: 1.6±0.1 g/cm3
  • Boiling Point: 879.3±65.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 295.2±27.8 °C

Topoisomerase II inhibitor 16

Topoisomerase II inhibitor 16 (compound CT3) is a selective, orally active, brain-penetrant and irreversible trypanosomal topoisomerase II inhibitor by stabilizing double-stranded DNA:enzyme cleavage complexes. Topoisomerase II inhibitor 16 has the potential for Chagas disease research[1].

  • CAS Number: 2403729-27-9
  • MF: C19H12F4N6O
  • MW: 416.33
  • Catalog: Topoisomerase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Topotecan hydrochloride hydrate

Topotecan hydrochloride hydrate is an orally active and potent Topoisomerase I inhibitor. Topotecan hydrochloride hydrate induces cell cycle arrest in G0/G1 and S phases and promotes apoptosis. Topotecan hydrochloride hydrate shows anticancer activity[1].

  • CAS Number: 1044663-62-8
  • MF: C23H26ClN3O6
  • MW: 475.92
  • Catalog: Apoptosis
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Proscillaridin A

Proscillaridin A is a potent poison of topoisomerase I/II activity with IC50 values of 30 nM and 100 nM, respectively[1].

  • CAS Number: 466-06-8
  • MF: C30H42O8
  • MW: 530.650
  • Catalog: Topoisomerase
  • Density: 1.3±0.1 g/cm3
  • Boiling Point: 716.7±60.0 °C at 760 mmHg
  • Melting Point: 233ºC
  • Flash Point: 232.6±26.4 °C

erythro-Austrobailignan-6

erythro-Austrobailignan-6 is an orally active anti-cancer agent. erythro-Austrobailignan-6 inhibits DNA topoisomerase I and II activity. erythro-Austrobailignan-6 induces cell apoptosis and increases phosphorylation of p38 and JNK[1][2].

  • CAS Number: 114127-24-1
  • MF: C20H24O4
  • MW: 328.4
  • Catalog: Apoptosis
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Topoisomerase II inhibitor 9

Topoisomerase II inhibitor 9 (Compound 19b) is a Topo II inhibitor with an IC50 of 0.97 μM. Topoisomerase II inhibitor 9 is also a classical DNA-intercalator with an IC50 of 43.51 μM. Topoisomerase II inhibitor 9 arrests the cell cycle at the G2/M phase and induces apoptosis in Hep G‐2 cells[1].

  • CAS Number: 2413901-61-6
  • MF: C22H17N7O3S2
  • MW: 491.55
  • Catalog: Apoptosis
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Topoisomerase II inhibitor 7

Topoisomerase II inhibitor 7 (compound 3a) is a potent inhibitor of topoisomerase II alpha subtype, with an IC50 of 3.19 μM. Topoisomerase II inhibitor 7 can induce cell cycle arrest and apoptosis[1].

  • CAS Number: 2697171-03-0
  • MF: C32H28BrN5O5S
  • MW: 674.56
  • Catalog: Apoptosis
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Murrayanol

Murrayanol is a natural carbazole alkaloid with a variety of biological activities. Murrayanol shows anti-inflammatory, topoisomerase I and topoisomerase II (Topoisomerase) inhibition activities. Murrayanol also as a mosquitocidal and antimicrobial[1].

  • CAS Number: 144525-81-5
  • MF: C24H29NO2
  • MW: 363.49300
  • Catalog: Bacterial
  • Density: 1.113±0.06 g/cm3(Predicted)
  • Boiling Point: 570.9±50.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: N/A

Ac-Exatecan

Ac-Exatecan is acetylated Exatecan (HY-13631). Exatecan (DX-8951) is a common toxin component in ADC preparation (ADC Cytotoxin) and an inhibitor of DNA topoisomerase I (IC50=2.2 μM)[1].

  • CAS Number: 2922852-48-8
  • MF: C26H24FN3O5
  • MW: 477.48
  • Catalog: ADC Cytotoxin
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Irinotecan hydrochloride

Irinotecan hydrochloride is a water soluble topoisomerase I inhibitor mainly used to treat colon cancer and rectal cancer.

  • CAS Number: 100286-90-6
  • MF: C33H39ClN4O6
  • MW: 623.139
  • Catalog: Autophagy
  • Density: N/A
  • Boiling Point: 257 °C
  • Melting Point: 250-256°C (dec.)
  • Flash Point: 482ºC

DRF-1042

DRF-1042 is an orally active camptothecin analog, which acts to inhibit DNA topoisomerase I. DRF-1042 shows good anticancer activity against a panel of human cancer cell lines[1][2].

  • CAS Number: 200619-13-2
  • MF: C22H20N2O6
  • MW: 408.404
  • Catalog: Topoisomerase
  • Density: 1.5±0.1 g/cm3
  • Boiling Point: 844.6±65.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 464.6±34.3 °C

Idarubicin

Idarubicin is an orally active and potent anthracycline antileukemic agent. Idarubicin inhibits the topoisomerase II interfering with the replication of DNA and RNA transcription. Idarubicin shows induction of DNA damage. Idarubicin inhibits DNA synthesis and of c-myc expression. Idarubicin inhibits the growth of bacteria and yeasts[1][2][3][4][5].

  • CAS Number: 58957-92-9
  • MF: C26H27NO9
  • MW: 497.494
  • Catalog: Bacterial
  • Density: 1.6±0.1 g/cm3
  • Boiling Point: 725.4±60.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 392.5±32.9 °C

Topoisomerase I inhibitor 7

Topoisomerase I inhibitor 7 (Compound 8) is a potent inhibitor of Topoisomerase I. Topoisomerase I inhibitor 7 significantly inhibits tumor growth (up to 79%) and increases the lifespan (153%) of mice bearing P388 lymphoma transplants. Topoisomerase I inhibitor 7 indicates prospects for further search of new antitumor drug candidates among the heteroarene-fused anthraquinones[1].

  • CAS Number: 2408639-81-4
  • MF: C22H19N3O5
  • MW: 405.40
  • Catalog: Topoisomerase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

RPR121056-d3

RPR121056-d3 is the deuterium labeled RPR121056. RPR121056 is a metabolite of Irinotecan (CPT-11), which is generated by CYP3A4. Irinotecan (CPT-11) is an antineoplastic agent that inhibits topoisomerase type I, causing cell death, and is widely used in the treatment of colorectal cancer. Irinotecan also directly inhibits AChE[1][2].

  • CAS Number: 1217816-76-6
  • MF: C33H35D3N4O8
  • MW: 621.70
  • Catalog: Topoisomerase
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

TP0480066

TP0480066 is a selective topoisomerase II inhibitor with IC50s of 1.10 and 62.89 nM for DNA gyrase and topo IV, respectively. TP0480066 shows good activity of againsting various bacterial species including drug-resistant strains. TP0480066 also exhibits potent inhibitory activity to N. gonorrhoeae, can be used in study of gonorrhea[1][2].

  • CAS Number: 2245693-15-4
  • MF: C18H14FN3O5
  • MW: 371.32
  • Catalog: Bacterial
  • Density: N/A
  • Boiling Point: N/A
  • Melting Point: N/A
  • Flash Point: N/A

Irinotecan

Irinotecan is a water soluble topoisomerase I inhibitor, preventing religation of the DNA strand by binding to topoisomerase I-DNA complex.

  • CAS Number: 97682-44-5
  • MF: C33H38N4O6
  • MW: 586.678
  • Catalog: Autophagy
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: 873.4±65.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 482.0±34.3 °C

Belotecan

Belotecan (CKD-602 free base) is a DNA topoisomerase I inhibitor. Belotecan induces cell apoptosis and cell-cycle arrest. Belotecan is a camptothecin analogue with anti-tumor effects, it can be used for the research of cancer[1].

  • CAS Number: 256411-32-2
  • MF: C25H27N3O4
  • MW: 433.500
  • Catalog: Apoptosis
  • Density: 1.4±0.1 g/cm3
  • Boiling Point: 772.4±60.0 °C at 760 mmHg
  • Melting Point: N/A
  • Flash Point: 420.9±32.9 °C

Epirubicin hydrochloride

Epirubicin (hydrochloride) is a semisynthetic L-arabino derivative of doxorubicin, and an antineoplastic agent by inhibiting Topoisomerase.

  • CAS Number: 56390-09-1
  • MF: C27H30ClNO11
  • MW: 579.980
  • Catalog: Topoisomerase
  • Density: 1.61g/cm3
  • Boiling Point: 810.3ºC at 760 mmHg
  • Melting Point: 185ºC dec
  • Flash Point: 443.8ºC